Monocyte activation in response to polyethylene glycol hydrogels grafted with RGD and PHSRN separated by interpositional spacers of various lengths
Monocyte activation in response to polyethylene glycol hydrogels grafted with RGD and PHSRN separated by interpositional spacers of various lengths
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DOI：
10.1002/jbm.a.31270
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发表时间：
2007-12-01
影响因子：
4.9
通讯作者：
Kao, Weiyuan John
中科院分区：
文献类型：
--
作者：
Schmidt, David Richard;Kao, Weiyuan John
聚乙二醇 (PEG) 通常被认为是一种“隐形”聚合物，能够抵抗蛋白质吸附和细胞粘附。推而广之，PEG 有望限制宿主反应。单核细胞衍生的巨噬细胞在炎症中发挥着不可或缺的作用，因此它们对材料的反应可能决定宿主对生物材料的整体反应。在本研究中，将单核细胞与光聚合 PEG 水凝胶相互作用后的反应与标准组织培养聚苯乙烯 (TCPS) 的反应进行了比较。此外，使用嫁接到 PEG 水凝胶上的具有不同间隔长度的肽评估了 RGD 和 PHSRN 之间的间隔（相应的纤连蛋白（FN）协同序列）的影响。仅 PEG 水凝胶上的单核细胞粘附密度与 TCPS 相当；然而，与 TCPS 相比，单核细胞与纯 PEG 水凝胶相互作用后，促炎分子白介素 1 β (IL-1 β)、肿瘤坏死因子 α (TNF-α) 和粒细胞巨噬细胞集落刺激因子 (GM-CSF) 的分泌显着增加。所有表面的基质金属蛋白酶 2 (MMP-2) 浓度相似，而基质金属蛋白酶 9 (MMP-9) 和 FN 浓度均高于所有底物的测定范围。与 PHSRNG(6)RGD 相比，PHSRNG(13)RGD 移植基质上的细胞密度更高，但与单独的 RGD 相比，两种序列都没有增加细胞密度。尽管蛋白质浓度有时会随不同肽而变化，但与 TCPS 和纯 PEG 水凝胶之间的表面效应相比，这种变化很小。本研究探讨了 PEG 和 FN 衍生肽对单核细胞活化的作用。 (C) 2007 年 Wiley 期刊公司。
Polyethylene glycol (PEG) is often cited as a "stealth" polymer, capable of resisting both protein adsorption and cell adhesion. By extension, PEG would then be expected to limit the host response. Monocyte-derived macrophages play an integral role in inflammation, and thus their response to a material can potentially dictate the overall host response to a biomaterial. In the present study, monocyte responses following interaction with a photopolymerized PEG hydrogel were compared with those from standard tissue culture polystyrene (TCPS). Additionally, the effect of the spacing between RGD and PHSRN, the corresponding synergy sequence on fibronectin (FN), was evaluated using peptides with differing spacer lengths grafted to the PEG hydrogel. Monocyte adherent density on the PEG-only hydrogel was comparable with that of TCPS; however, the secretion of the proinflammatory molecules interleukin-1 beta (IL-1 beta), tumor necrosis factor-a (TNF-alpha), and granulocyte-macrophage colony stimulating factor (GM-CSF) increased dramatically following monocyte interaction with PEG-only hydrogels as compared with TCPS. The matrix metalloproteinase-2 (MMP-2) concentration was similar for all surfaces, while both the matrix metalloproteinase-9 (MMP-9) and FN concentrations were above the range of the assay for all substrates. Cell density was higher on the PHSRNG(13)RGD grafted substrate as compared with PHSRNG(6)RGD, but neither sequence increased cell density versus RGD alone. Although protein concentration did sometimes vary with different peptides, this variation was minimal in comparison with the surface effects between TCPS and the PEG-only hydrogel. This study explores the roles of PEG and FN-derived peptides on monocyte activation. (C) 2007 Wiley Periodicals, Inc.