Bioluminescence of the arm light organs of the luminous squid Watasenia scintillans
Bioluminescence of the arm light organs of the luminous squid Watasenia scintillans
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DOI：
10.1016/j.bbagen.2008.01.016
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发表时间：
2008-05-01
影响因子：
3
通讯作者：
Shimomura, Osamu
中科院分区：
文献类型：
--
作者：
Teranishi, Katsunori;Shimomura, Osamu
鱿鱼Watasenia flintillans从众多的发光体中发出蓝色光。根据Tsuji[F.I.Tsuji]，膜结合的荧光素酶催化的生物发光反应在“萤火虫鱿鱼”，Watasenia scintillans，Biochim。生物群落。]，手臂发光器的发光是由依赖于ATP的反应引起的，该反应涉及到镁(2+)、二硫氰菊酯(荧光素)和一个不稳定的膜结合的荧光素酶。我们在高浓度蔗糖存在下稳定并部分纯化了荧光素酶，得到了平均大小为0.6-2微米的颗粒。详细研究了颗粒型荧光素酶催化的二硫氰菊酯依赖于ATP的发光反应。发光反应的最适温度约为5℃。在该发光体系中，二硫氰菊酯是一种严格特定的底物；其结构的任何改变都会导致其发光能力的严重损失。光发射体是二硫酸腔肠酰胺的酰胺阴离子形式的激发态。计算得到二硫代乙酰乙酸乙酯的量子产率为0.36。三磷酸腺苷可以被三磷酸腺苷-伽马-S所取代，但不能被任何其他被测试的类似物所取代。发光反应中产生的AMP量远小于肠肠胺二硫酸盐，表明渡葵生物发光的反应机理不涉及作为中间体的腺基荧光素的形成。(C)2008爱思唯尔B.V.保留所有权利。
The squid Watasenia scintillans emits blue light from numerous photophores. According to Tsuji [F.I. Tsuji, Bioluminescence reaction catalyzed by membrane-bound luciferase in the "firefly squid", Watasenia scintillans, Biochim. Biophys. Acta 1564 (2002) 189-197.], the luminescence from arm light organs is caused by an ATP-dependent reaction involving Mg(2+), coelenterazine disulfate (luciferin), and an unstable membrane-bound luciferase. We stabilized and partially purified the luciferase in the presence of high concentrations of sucrose, and obtained it as particulates (average size 0.6-2 mu m). The ATP-dependent luminescence reaction of coelenterazine disulfate catalyzed by the particulate luciferase was investigated in detail. Optimum temperature of the luminescence reaction is about 5 degrees C. Coelenterazine disulfate is a strictly specific substrate in this luminescence system; any modification of its structure resulted in a very heavy loss in its light emission capability. The light emitter is the excited state of the amide anion form of coelenteramide disulfate. The quantum yield of coelenterazine disulfate is calculated at 0.36. ATP could be replaced by ATP-gamma-S, but not by any other analogues tested. The amount of AMP produced in the luminescence reaction was much smaller than that of coelenteramide disulfate, suggesting that the reaction mechanism of the Watasenia bioluminescence does not involve the formation of adenyl luciferin as an intermediate. (C) 2008 Elsevier B.V. All rights reserved.