Personal cytometers: slow flow or no flow?
Personal cytometers: slow flow or no flow?
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个人细胞仪：慢流还是无流？
DOI：
10.1002/cyto.a.20284
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Perlmutter,NancyG
中科院分区：
文献类型：
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作者：
Shapiro,HowardM;Perlmutter,NancyG
背景资料：虽然有厂商乐观地将价格在美国4万元左右的仪器描述为“个人血细胞仪”，但以个人电脑为类比，真正的“个人“血细胞仪的目标价格应该在5000元以下。由于这种装置在发展中国家和发达国家的细胞分离学中都有广泛的应用，因此似乎有必要考虑其技术和经济可行性。研究方法：使用分辨率目标和固定在过滤器和/或载玻片上的各种荧光珠标准品，我们评估了高强度LED作为荧光激发源，相对便宜的CCD相机作为检测器，以及35 mm相机镜头和塑料低倍显微镜光学器件用于简单，廉价的低分辨率成像细胞仪中的光收集。结果如下：测试的组件可以组合以产生能够检测少于10，000个细胞相关荧光标记分子的仪器，因此适用于广泛的细胞计数任务。结论：考虑到对光源、检测器、光学、机械、电子和数据分析硬件和软件的要求，以及目前可用的组件，与流式细胞仪相比，达到所需的5，000价格点的动物细胞仪应该更容易。© 2006 InternationalSocietyforAnalyticalCytology
Background: Although some manufacturers have optimistically described instruments with prices in the US 40,000rangeas“personalcytometers”,analogywiththepersonalcomputersuggeststhatthetargetpriceforatrue“personal”cytometershouldbeunder 5,000. Since such an apparatus could find a wide range of applications in cytomics in both developing and developed countries, it seemed desirable to consider its technical and economic feasibility. Methods: Using resolution targets and a variety of fluorescent bead standards immobilized on filters and/or slides, we evaluated high‐intensity LEDs as fluorescence excitation sources, relatively inexpensive CCD cameras as detectors, and 35 mm camera lenses and plastic low‐power microscope optics for light collection in a simple, inexpensive low‐resolution imaging cytometer. Results: The components tested could be combined toproduce an instrument capable of detecting fewer than 10,000 molecules of cell‐associated fluorescent label, and thus applicable to a broad range of cytometric tasks. Conclusions: Given the requirements for light sources, detectors, optics, mechanics, electronics and data analysis hardware and software, and the components presently available, it should be easier to reach the desired 5,000pricepointwithanimagecytometerthanwithaflowcytometer.©2006InternationalSocietyforAnalyticalCytology