Inhibition of a DNA-helicase by peptide nucleic acids.
Inhibition of a DNA-helicase by peptide nucleic acids.
复制标题
肽核酸对 DNA 解旋酶的抑制。
DOI：
10.1093/nar/27.2.551
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发表时间：
1999
影响因子：
14.9
通讯作者：
Lebleu,B
中科院分区：
文献类型：
--
作者：
Bastide,L;Boehmer,PE;Villani,G;Lebleu,B
双肽核酸 (bis-PNA) 结合导致 DNA 双链体中互补同型嘌呤片段上的链置换形成 D 环。完整细胞中的转录和复制是由涉及聚合酶以外的多种蛋白质的多酶复合物介导的。迄今为止，已经研究了双-PNA 形成的高度稳定的钳结构的行为，即其阻止 RNA 聚合酶的能力。 DNA 解旋酶对它们的识别和加工却很少受到关注。在本报告中，我们研究了 bis-PNA 对已明确表征的 I 型单纯疱疹 UL9 蛋白 DNA 解旋酶活性的抑制作用。双 PNA 显着抑制 UL9 对合成底物的解旋，并且添加 ICP8 蛋白（可增加 UL9 持续合成能力）并不能缓解这种抑制。
Bis-peptide nucleic acid (bis-PNA) binding results in D-loop formation by strand displacement at complementary homopurine stretches in DNA duplexes. Transcription and replication in intact cells is mediated by multienzymatic complexes involving several proteins other than polymerases. The behaviour of the highly stable clamp structure formed by bis-PNAs has thus far been studied with respect to their capacity to arrest RNA polymerases. Little attention has been given to their recognition and processing by DNA helicases. In this report we have investigated the inhibitory effect of a bis-PNA on the DNA-helicase activity of the well characterized herpes simplex type I UL9 protein. Unwinding by UL9 of a synthetic substrate is significantly inhibited by a bis-PNA and the addition of the ICP8 protein, which increases UL9 processivity, does not relieve this inhibition.