Effects of ECM protein micropatterns on the migration and differentiation of adult neural stem cells.
Effects of ECM protein micropatterns on the migration and differentiation of adult neural stem cells.
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DOI：
10.1038/srep13043
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发表时间：
2015-08-12
影响因子：
4.6
通讯作者：
Nam Y
中科院分区：
文献类型：
--
作者：
Joo S;Yeon Kim J;Lee E;Hong N;Sun W;Nam Y
成体神经干细胞（aNSCs）的迁移和分化被认为受到干细胞生态位中细胞外基质（ECM）蛋白空间分布的强烈影响。体外培养平台涉及ECM蛋白的特定空间分布，可以为更好地理解ECM蛋白空间格局中的aNSC行为提供新的工具。在这项工作中，我们应用软光刻技术设计了简单和可重复的层粘连蛋白(LN)-聚赖氨酸细胞培养底物，并研究了aNSC如何响应层粘连蛋白的不同空间分布，层粘连蛋白是aNSC生态位中富集的ECM蛋白之一。我们发现，aNSC倾向于迁移和附着于LN条纹，aNSC衍生的神经元和星形胶质细胞对LN条纹的运动性有显著差异。通过改变LN条纹的间距，我们能够控制神经元和星形胶质细胞的排列。据我们所知，这是第一次利用表面微模式研究aNSCs对ECM蛋白（LN）和细胞粘附合成聚合物（PDL）的细胞差异反应。我们的发现将对星形胶质细胞-神经元相互作用以及ecm -干细胞相互作用提供更深入的理解。
The migration and differentiation of adult neural stem cells (aNSCs) are believed to be strongly influenced by the spatial distribution of extracellular matrix (ECM) proteins in the stem cell niche. In vitro culture platform, which involves the specific spatial distribution of ECM protein, could offer novel tools for better understanding of aNSC behavior in the spatial pattern of ECM proteins. In this work, we applied soft-lithographic technique to design simple and reproducible laminin (LN)-polylysine cell culture substrates and investigated how aNSCs respond to the various spatial distribution of laminin, one of ECM proteins enriched in the aNSC niche. We found that aNSC preferred to migrate and attach to LN stripes, and aNSC-derived neurons and astrocytes showed significant difference in motility towards LN stripes. By changing the spacing of LN stripes, we were able to control the alignment of neurons and astrocytes. To the best of our knowledge, this is the first time to investigate the differential cellular responses of aNSCs on ECM protein (LN) and cell adhesive synthetic polymer (PDL) using surface micropatterns. Our findings would provide a deeper understanding in astrocyte-neuron interactions as well as ECM-stem cell interactions.