Regeneration and the immune system I. In vitro and in vivo activation of lymphocytes by liver regeneration and the role of Kupffer cells in stimulation
Regeneration and the immune system I. In vitro and in vivo activation of lymphocytes by liver regeneration and the role of Kupffer cells in stimulation
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再生与免疫系统 I. 肝再生对淋巴细胞的体外和体内激活以及库普弗细胞的刺激作用
DOI：
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发表时间：
1983
影响因子：
5.4
通讯作者：
Y. Kimura
中科院分区：
文献类型：
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作者：
Seiko Miyahara;K. Yokomuro;Hidemi Takahashi;Y. Kimura
当同基因淋巴细胞和丝裂霉素C处理的再生肝细胞（由部分肝切除小鼠制备）一起培养时，体外DNA合成被激活（sMLDNA：同基因混合肝切除肝细胞淋巴细胞培养物）。Ia+枯否细胞在应答中作为刺激因子发挥重要作用，因为再生肝细胞的刺激活性通过抗Ia单克隆抗体加补体预处理或通过从其中去除枯否细胞而丧失。淋巴细胞在部分肝切除后的肝再生过程中也被激活。当从肝切除小鼠制备的淋巴细胞与再生肝细胞一起培养时，淋巴细胞受到刺激，以典型的二次免疫应答（二次sML）的方式加速其DNA合成。在原发性sML中，应答细胞主要是Lyt-1+，而在继发性sML中，它们主要是Lyt-2+。讨论了在sML过程中主要应答细胞的Lyt表型从Lyt-1变为Lyt-2的机制。
When syngeneic lymphocytes and mitomycin C‐treated regenerating liver cells, prepared from partially hepatectomized mice, are cultured together, the in vitro DNA synthesis is activated (sMLHLR: syngeneic mixed hepatectomized‐liver cell‐lymphocyte culture). The Ia+ Kupffer cells play an important role as stimulators in the response, since the stimulating activity of regenerating liver cells is lost either by the pretreatment with anti‐Ia monoclonal antibody plus complement or by the removing Kupffer cells from them. The lymphocytes are also activated in vivo during liver regeneration following partial hepatectomy. When lymphocytes, prepared from hepatectomized mice, are cultured with regenerating liver cells, the lymphocytes are stimulated to accelerate their DNA synthesis in a manner typical of the secondary immune responses (secondary sMLHLR). In primary sMLHLR, the responder cells are mainly Lyt‐1+, whereas, in secondary sMLHLR, they are mainly Lyt‐2+. The mechanism of changing the Lyt phenotype of major responder cells from Lyt‐1 to Lyt‐2 during sMLHLR is discussed.
针对 H-2K 和 H-2D 差异的初级混合白细胞反应需要 Ia 阳性刺激细胞，但在次级混合白细胞反应中不需要。
DOI：
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发表时间：
1981
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Minami,M;Shreffler,DC
通讯作者：
Shreffler,DC