Immunohistochemical detection of WT1 protein in a variety of cancer cells
Immunohistochemical detection of WT1 protein in a variety of cancer cells
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DOI：
10.1038/modpathol.3800588
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发表时间：
2006-06-01
期刊：
影响因子：
7.5
通讯作者：
Aozasa, K
中科院分区：
文献类型：
--
作者：
Nakatsuka, S;Oji, Y;Aozasa, K
WT 1最初被鉴定为参与肾母细胞瘤发展的肿瘤抑制因子。最近，WT 1的致癌特性已在各种血液恶性肿瘤和实体瘤中得到证实。由于WT 1已被确定为癌症免疫治疗的分子靶点，因此肿瘤细胞中WT 1的免疫组织化学检测已成为常规实践的重要组成部分。在本研究中，WT 1的表达进行了检查，在494例人类癌症，包括肿瘤的胃肠道和胰胆系统，泌尿道，男性和女性生殖器官，乳腺，肺，脑，皮肤，软组织和骨免疫组化使用多克隆（C-19）和单克隆（6 F-H2）抗体对WT 1蛋白。C-19和6 F-H2染色分别见于35-100和5-88%的每种肿瘤病例。WT 1阳性肿瘤包括胃、前列腺、胆道和泌尿系统肿瘤以及恶性黑色素瘤。大多数阳性病例在胞浆中呈弥漫性或颗粒状染色，而卵巢肿瘤和促结缔组织增生性小圆细胞肿瘤常呈核染色。胶质母细胞瘤、一些软组织肉瘤、骨肉瘤和皮肤恶性黑色素瘤与其他肿瘤相比，显示出极强的细胞质染色。Western blot分析显示，WT 1蛋白主要表达在两例肺腺癌的肿瘤细胞的胞浆中，支持使用免疫组化的胞浆内染色的WT 1。常规处理的组织切片的免疫组化检测可以提供有意义的信息，在癌细胞中的表达WT 1。
WT1 was first identified as a tumor suppressor involved in the development of Wilms' tumor. Recently, oncogenic properties of WT1 have been demonstrated in various hematological malignancies and solid tumors. Because WT1 has been identified as a molecular target for cancer immunotherapy, immunohistochemical detection of WT1 in tumor cells has become an essential part of routine practice. In the present study, the expression of WT1 was examined in 494 cases of human cancers, including tumors of the gastrointestinal and pancreatobiliary system, urinary tract, male and female genital organs, breast, lung, brain, skin, soft tissues and bone by immunohistochemistry using polyclonal (C-19) and monoclonal (6F-H2) antibodies against WT1 protein. Staining for C-19 and 6F-H2 was found in 35-100 and 5-88% of the cases of each kind of tumor, respectively. WT1-positive tumors included tumor of the stomach, prostate, and biliary and urinary systems, and malignant melanomas. A majority of the positive cases showed diffuse or granular staining in the cytoplasm, whereas ovarian tumors and desmoplastic small round cell tumors frequently showed nuclear staining. Glioblastomas, some of soft tissue sarcomas, osteosarcomas, and malignant melanomas of the skin showed extremely strong cytoplasmic staining as compared with other tumors. Western blot analysis showed that WT1 protein was predominantly expressed in the cytoplasm of the tumor cells in two cases of lung adenocarcinoma, supporting the intracytoplasmic staining for WT1 using immunohistochemistry. Immunohistochemical detection with routinely processed histologic sections could provide meaningful information on the expression of WT1 in cancer cells.