Organelle acidification is important for localisation of vacuolar proteins in Saccharomyces cerevisiae.
Organelle acidification is important for localisation of vacuolar proteins in Saccharomyces cerevisiae.
复制标题
细胞器酸化对于酿酒酵母中液泡蛋白的定位很重要。
DOI：
10.1007/s00709-013-0510-2
复制
发表时间：
2013
期刊：
影响因子：
2.9
通讯作者：
Matsumoto R
中科院分区：
文献类型：
--
作者：
Suzuki K;Nakamura S;Morimoto M;Fujii K;Noda NN;Inagaki F;Ohsumi Y.;鈴木邦律他;Matsumoto R
液泡和其他酸性细胞器中的酸性环境对于真核细胞中的许多细胞过程是重要的。在这项研究中，我们全面调查的液泡蛋白定位在酿酒酵母细胞器酸化的作用。在用空泡H+-ATP酶（V-ATP酶）的特异性抑制剂康卡霉素A处理抑制酸性隔室的酸化后，我们研究了预测定位于空泡腔或空泡膜上的GFP融合蛋白的定位。在73种蛋白质中，有19种蛋白质改变了它们在细胞质区域的定位。使用图像处理程序CalMorph定量评价定位变化。离域蛋白质包括液泡水解酶、V-ATP酶亚基、转运蛋白和膜生物合成酶，以及蛋白质转运所需的蛋白质。这些结果表明，许多变化发生在液泡蛋白的本地化后的酸性隔间的酸化损失。
The acidic environments in the vacuole and other acidic organelles are important for many cellular processes in eukaryotic cells. In this study, we comprehensively investigated the roles of organelle acidification in vacuolar protein localisation inSaccharomyces cerevisiae. After repressing the acidification of acidic compartments by treatment with concanamycin A, a specific inhibitor of vacuolar H+-ATPase (V-ATPase), we examined the localisation of GFP-fused proteins that were predicted to localise in the vacuolar lumen or on the vacuolar membrane. Of the 73 proteins examined, 19 changed their localisation to the cytoplasmic region. Localisation changes were evaluated quantitatively using the image processing programme CalMorph. The delocalised proteins included vacuolar hydrolases, V-ATPase subunits, transporters and enzymes for membrane biogenesis, as well as proteins required for protein transport. These results suggest that many alterations in the localisation of vacuolar proteins occur after loss of the acidification of acidic compartments.