RNA LOCALIZATION ALONE THE ANTEROPOSTERIOR AXIS OF THE DROSOPHILA OOCYTE REQUIRES PKA-MEDIATED SIGNAL-TRANSDUCTION TO DIRECT NORMAL MICROTUBULE ORGANIZATION
RNA LOCALIZATION ALONE THE ANTEROPOSTERIOR AXIS OF THE DROSOPHILA OOCYTE REQUIRES PKA-MEDIATED SIGNAL-TRANSDUCTION TO DIRECT NORMAL MICROTUBULE ORGANIZATION
复制标题
DOI：
10.1101/gad.8.24.2986
复制
发表时间：
1994-12-15
影响因子：
10.5
通讯作者：
KALDERON, D
中科院分区：
文献类型：
--
作者：
LANE, ME;KALDERON, D
微管极性已被牵连的基础上极化定位的形态发生决定因素，指定的前后轴在果蝇卵母细胞。我们描述了影响蛋白激酶A（PKA）的突变，该突变在生殖细胞系中起作用，破坏微管分布和RNA定位沿着该轴。在正常卵母细胞中，微管成核的位置从后移到前，紧接着bicoid和oskar RNA的极化定位。在PKA缺陷的卵母细胞中，在此过渡期间存在后部微管，奥斯卡RNA不能在后部积累，而bicoid RNA在卵母细胞的两端积累。先前报道的Notch和Delta突变体的类似RNA错误定位模式表明PKA转导由位于后方的卵泡细胞发送的微管重组信号。
Microtubule polarity has been implicated as the basis for polarized localization of morphogenetic determinants that specify the anteroposterior axis in Drosophila oocytes. We describe mutations affecting Protein Kinase A (PKA) that act in the germ line to disrupt both microtubule distribution and RNA localization along this axis. In normal oocytes, the site of microtubule nucleation shifts from posterior to anterior immediately prior to polarized localization of bicoid and oskar RNAs. In PKA-deficient oocytes, posterior microtubules are present during this transition, oskar RNA fails to accumulate at the posterior, and bicoid RNA accumulates at both ends of the oocyte. Similar RNA mislocalization patterns previously reported for Notch and Delta mutants suggest that PKA transduces a signal for microtubule reorganization that is sent by posteriorly located follicle cells.