Hydrodynamic characterization within a spinner flask and a rotary wall vessel for stem cell culture
Hydrodynamic characterization within a spinner flask and a rotary wall vessel for stem cell culture
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DOI：
10.1016/j.bej.2020.107533
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发表时间：
2020-04-15
影响因子：
3.9
通讯作者：
Princevac, Marko
中科院分区：
文献类型：
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作者：
Ghasemian, Masoud;Layton, Carys;Princevac, Marko
搅拌悬浮培养正在成为扩增人类多能干细胞 (hPSC) 的流行方法。虽然搅拌产生足够的流体运动来提升细胞并促进质量转移（营养物、溶解气体和代谢废物），但过度搅拌可能会施加对细胞生长有害的水动力。在本研究中，进行计算流体动力学 (CFD) 模拟，首先研究旋转烧瓶（实验室中常用的搅拌悬浮培养容器）中流体流动的流体动力学特性。获得了不同叶轮速度下的流型和剪切应力分布以及柯尔莫哥洛夫长度尺度。作者之前的实验研究中测量的 Kolmogorov 长度尺度和 hPSC 聚集体大小的比较表明，两者之间存在很强的相关性。除了产生复杂和瞬态湍流的旋转烧瓶之外，本研究还研究了一种新开发的旋转壁容器，该容器被设计用于产生层流、圆形库埃特流以控制剪切应力。 CFD 模拟显示，与旋转烧瓶中的流动相比，流动明显更加均匀和均匀，表明旋转壁容器是研究悬浮液中剪切应力对 hPSC 的作用的合适培养容器。
Stirred suspension culture is becoming a popular method for expanding human pluripotent stem cells (hPSCs). While stirring generates adequate fluid motions to lift the cells and facilitates mass transfers (of nutrients, dissolved gases, and metabolic wastes), excessive stirring could impose hydrodynamic forces deleterious for the growth of the cells. In this study, computational fluid dynamics (CFD) simulations were performed to first investigate hydrodynamic characteristics of fluid flows in a spinner flask, a common stirred suspension culture vessel used in laboratories. Flow patterns and distributions of shear stresses and the Kolmogorov length scales at varying impeller speeds were obtained. Comparison of the Kolmogorov length scales and sizes of hPSC aggregates, measured in the authors' previous experimental study, showed a strong correlation between the two. In addition to the spinner flask which generated complex and transient turbulent flows, this study investigated a newly developed rotary wall vessel that had been designed to produce laminar, circular Couette flows in order to control shear stress. CFD simulations revealed significantly more uniform and homogeneous flows compared to those in the spinner flask, suggesting that the rotary wall vessel is a suitable culture vessel to investigate roles of shear stress on hPSCs in suspension.