Cellulase production from a new strain Acremonium strictum isolated from the Brazilian Biome using different substrates
Cellulase production from a new strain Acremonium strictum isolated from the Brazilian Biome using different substrates
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DOI：
10.1016/j.biortech.2012.10.034
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发表时间：
2013-01-01
影响因子：
11.4
通讯作者：
Maugeri-Filho, Francisco
中科院分区：
文献类型：
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作者：
Goldbeck, Rosana;Ramos, Mayla M.;Maugeri-Filho, Francisco
本研究的目的是评估使用不同底物的巴西生物群系中分离的枝顶孢菌strictum的纤维素分解酶的生产。最初使用商业底物进行发酵，所述商业底物包括微晶纤维素和羧甲基纤维素。随后使用木质纤维素生物质进行发酵：以不同强度预处理的甘蔗渣。发酵在摇瓶中以150 rpm、30 ℃进行240 h。测定了四种酶的活性：CMCase、FPase、纤维二糖酶和β-葡萄糖苷酶。在所用的基质中，结果表明，甘蔗渣提交温和的预处理是诱导微生物产生更大的纤维素分解活性。利用该底物对A. strictum。(C)2012爱思唯尔有限公司保留所有权利。
The objective of the present study was to evaluate the production of cellulolytic enzymes by Acremonium strictum isolated from Brazilian Biome using different substrates. Fermentations were initially carried out using commercial substrates, including microcrystalline cellulose (AVICEL (R) and SERVACEL (R)) and carboxymethylcellulose (CMC). This was followed by fermentations performed using lignocellulosic biomass: sugarcane bagasse pretreated at different intensities. The fermentations were carried out in shakers at 150 rpm, 30 degrees C for 240 h. Four enzyme activities were determined: CMCase, FPase, cellobiase and beta-glucosidase. Among the substrates used, results showed that the sugarcane bagasse submitted to mild pretreatment was that which induced the microorganism to produce greater cellulolytic activities. This substrate was employed in the optimization study of cellulase production by A. strictum. (C) 2012 Elsevier Ltd. All rights reserved.