Resistance to endotoxin shock and reduced dissemination of gram-negative bacteria in CD14-deficient mice
Resistance to endotoxin shock and reduced dissemination of gram-negative bacteria in CD14-deficient mice
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DOI：
10.1016/s1074-7613(00)80254-x
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发表时间：
1996-04-01
期刊：
影响因子：
32.4
通讯作者：
Goyert, SM
中科院分区：
文献类型：
--
作者：
Haziot, A;Ferrero, E;Goyert, SM
内毒素休克是内毒素/LPS（革兰氏阴性菌的一种成分）激活免疫系统的结果。 CD14 是一种由单核细胞/巨噬细胞强烈表达的 GPI 锚定糖蛋白，是内毒素/LPS 的几种受体之一。在通过胚胎干细胞基因靶向产生的 CD14 缺陷小鼠中测试了 CD14 在细菌诱导和 LPS 诱导的休克中的作用。研究发现，CD14 缺陷小鼠对活革兰氏阴性细菌或 LPS 诱导的休克具有高度抵抗力。然而，在 LPS 或细菌浓度非常高的情况下，可以检测到通过非 CD14 受体的反应。令人惊讶的是，CD14 缺陷小鼠的菌血症水平也显着降低，这表明 CD14 在革兰氏阴性菌传播中发挥着意想不到的作用。
Endotoxin shock is the result of activation of the immune system by endotoxin/LPS, a component of Gram-negative bacteria. CD14, a GPI-anchored glycoprotein expressed strongly by monocyte/macrophages, is one of several receptors for endotoxin/LPS. The role of CD14 in bacterial-induced and LPS-induced shock was tested in CD14-deficient mice produced by gene targeting in embryonic stem cells. CD14-deficient mice were found to be highly resistant to shock induced by either live Gram-negative bacteria or LPS; however, at very high concentrations of LPS or bacteria, responses through non-CD14 receptors could be detected. Surprisingly, CD14-deficient mice also showed dramatically reduced levels of bacteremia, suggesting an unexpected role for CD14 in the dissemination of Gram-negative bacteria.