Peripheral blood lymphocyte subset repertoires are biased and reflect clinical features in patients with dermatomyositis.
Peripheral blood lymphocyte subset repertoires are biased and reflect clinical features in patients with dermatomyositis.
复制标题
外周血淋巴细胞亚群谱存在偏差，反映了皮肌炎患者的临床特征。
DOI：
10.1080/03009742.2018.1530371
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发表时间：
2018
影响因子：
2.1
通讯作者：
Kohsaka H
中科院分区：
文献类型：
--
作者：
Sasaki H;Takamura A;Kawahata K;Takashima T;Imai K;Morio T;Kohsaka H
目的：皮肌炎（DM）是一种累及肺部的特发性炎症性肌病。DM可能与发病机制中的异常T细胞和B细胞活化相关，因为T细胞和B细胞靶向治疗的有效性已得到证实。假设异常激活反映了淋巴细胞亚群库的偏差，我们的目的是阐明这些偏差，特别是与DM的临床特征有关。根据人类免疫学项目联盟标准化的免疫表型，未治疗的13名糖尿病患者，包括7名间质性肺病（ILD）患者，同时检测18例年龄匹配的健康献血员外周血淋巴细胞亚群比例。结果：DM患者的幼稚CD4+T细胞和幼稚B细胞较HD患者丰富，幼稚CD8+T细胞、中枢记忆CD8 + T细胞、效应记忆CD4+T细胞、Th1细胞、Tfh细胞和记忆B细胞较HD患者减少。当根据ILD的存在对患者进行亚组时，ILD患者的淋巴细胞亚群库导致所有偏倚淋巴细胞亚群比例的统计学差异。结论：DM患者外周血B细胞亚群存在偏倚，并与ILD的发生及DM的病情活动有关。
Objective: Dermatomyositis (DM) is an idiopathic inflammatory myopathy which often involves the lungs. DM is likely to be associated with aberrant T- and B-cell activation in the pathogenesis because of the proven effectiveness of T- and B-cell-targeted treatments. Assuming that the aberrant activation is reflected by biases in the lymphocyte subset repertoires, we aimed to elucidate these biases, especially in relation to clinical features of DM.Method: Based on the immunophenotyping standardized by the Human Immunology Project Consortium, untreated 13 DM patients, including seven patients with interstitial lung disease (ILD), and 18 age-matched healthy donors (HDs) were examined for proportions of peripheral blood lymphocyte subsets. Six DM patients were examined before and after successful induction of remission.Results: Naïve CD4+T cells and naïve B cells were more abundant, while there were fewer naïve CD8+T cells, central memory CD8+T cells, effector memory CD4+T cells, Th1 cells, Tfh cells, and memory B cells in DM patients than in HDs. When the patients were subgrouped according to the presence of ILD, the lymphocyte subset repertoires in the patients with ILD contributed to the statistical differences in all the biased lymphocyte subset proportions. After treatment, transitional B cells vanished and there was an increase in memory B cells.Conclusion: The lymphocyte subset repertoires in the DM patients were biased, and were associated with the presence of ILD and disease activity of DM.