CYSTOLYTIC EVENTS AND THE POSSIBLE ROLE OF GERMINAL CELLS IN METASTASIS IN CHRONIC ALVEOLAR HYDATIDOSIS
CYSTOLYTIC EVENTS AND THE POSSIBLE ROLE OF GERMINAL CELLS IN METASTASIS IN CHRONIC ALVEOLAR HYDATIDOSIS
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DOI：
10.1080/00034983.1983.11811742
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发表时间：
1983-01-01
影响因子：
--
通讯作者：
FAUCHER, M
中科院分区：
文献类型：
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作者：
ALIKHAN, Z;SIBOO, R;FAUCHER, M
从C57 BL/6 J和Balb/cJ小鼠中分离出2周龄和6周龄的泡状囊泡状棘球蚴，并用于研究由驻留的炎性细胞介导的溶囊事件。将这些囊肿的超微结构退行性变化与体外生长的囊肿（无炎性细胞）的超微结构进行比较。发现巨噬细胞、嗜酸性粒细胞和中性粒细胞结合于囊泡胚层的质膜层。囊肿表面炎性细胞释放的水解酶和嗜酸性粒细胞颗粒溶解了层压层，并在质膜上产生间隙。巨噬细胞主动吞噬崩解层的颗粒状基质。囊泡溶解导致6-17 μ m大小的胚泡细胞的释放，其具有带显著核仁的核、稀疏的内质网、高尔基复合体和椭圆形线粒体。根据组织学证据，认为生殖细胞可能是囊肿的祖细胞。在免疫功能低下的宿主中，生发细胞可能引起肺泡囊肿远处转移灶的可能性进行了讨论。
Two and six week old alveolar cysts of Echinococcus multilocularis were isolated from C57BL/6J and Balb/cJ mice and used to study the cystolytic events mediated by resident inflammatory cells. Ultramicroscopic degenerative changes in these cysts were compared with the ultrastructure of in vitro grown cysts (free of inflammatory cells). Macrophages, eosinophils and neutrophils were found to bind to the laminated layer of plasma membrane of the germinal layer of cysts. Hydrolytic enzymes and eosinophil granules released extracellularly from inflammatory cells at the cyst surface appear to dissolve the laminated layer and produce gaps in the plasma membrane. Macrophages actively phagocytose the granular ground substance of the disintegrated laminated layer. Cystolysis results in the release of germinal cells which measure 6-17 .mu.m, possess nuclei with prominent nucleoli, sparse endoplasmic reticulum, Golgi complexes and oval mitochondria. Based on histologic evidence it is believed that germinal cells may be the progenitors of cysts. The possibility that germinal cells may give rise to distant metastatic foci of alveolar cysts, in immunocompromised hosts, is discussed.