Evidence of bat origin for Menangle virus, a zoonotic paramyxovirus first isolated from diseased pigs
Evidence of bat origin for Menangle virus, a zoonotic paramyxovirus first isolated from diseased pigs
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DOI：
10.1099/vir.0.045385-0
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发表时间：
2012-12-01
影响因子：
3.8
通讯作者：
Wang, Lin-Fa
中科院分区：
文献类型：
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作者：
Barr, Jennifer A.;Smith, Craig;Wang, Lin-Fa
Menangle病毒（MenPV）是一种人畜共患副粘病毒，能够在猪和人类中引起疾病。它于1997年首次从澳大利亚新南威尔士州一个商业养猪场的死胎仔猪中分离出来，当时发生了生殖疾病的爆发。在澳大利亚的各种蝙蝠物种中检测到MenPV的中和抗体，果蝠被怀疑是导致猪爆发的病毒的来源。然而，以前试图从各种果蝠物种中分离MenPV的尝试证明是徒劳的。在这里，我们报告分离MenPV的尿液样本的黑狐蝠，狐蝠，使用改进的程序和新建立的蝙蝠细胞系的组合。蝙蝠分离株的核苷酸序列与猪分离株的核苷酸序列有94%相同。这一发现提供了强有力的证据支持这一假设，即MenPV在猪中的爆发起源于栖息在猪圈附近的蝙蝠中的病毒。
Menangle virus (MenPV) is a zoonotic paramyxovirus capable of causing disease in pigs and humans. It was first isolated in 1997 from stillborn piglets at a commercial piggery in New South Wales, Australia, where an outbreak of reproductive disease occurred. Neutralizing antibodies to MenPV were detected in various pteropid bat species in Australia and fruit bats were suspected to be the source of the virus responsible for the outbreak in pigs. However, previous attempts to isolate MenPV from various fruit bat species proved fruitless. Here, we report the isolation of MenPV from urine samples of the black flying fox, Pteropus alecto, using a combination of improved procedures and newly established bat cell lines. The nucleotide sequence of the bat isolate is 94% identical to the pig isolate. This finding provides strong evidence supporting the hypothesis that the MenPV outbreak in pigs originated from viruses in bats roosting near the piggery.