Human testis cDNAs identified by sera from infertile patients: a molecular biological approach to immunocontraceptive development.
Human testis cDNAs identified by sera from infertile patients: a molecular biological approach to immunocontraceptive development.
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通过不育患者血清鉴定的人类睾丸 cDNA：免疫避孕开发的分子生物学方法。
DOI：
10.1071/rd9940297
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Goldberg,E
中科院分区：
文献类型：
--
作者：
Liang,ZG;O'Hern,PA;Yavetz,B;Yavetz,H;Goldberg,E
从已知或疑似免疫性不育患者的血清中筛选人睾丸cDNA文库。通过北方印迹分析，总共59份血清检测到38个独特的cDNA插入片段，其中4个是睾丸特异性的。其中之一是睾丸特异性的钙蛋白酶抑制蛋白同种型。另外五个克隆，虽然不是睾丸特异性的，被发现是睾丸丰富。这些人血清鉴定的克隆的数量和类型提示免疫性不育的可能病因。睾丸特异性克隆将进一步表征以确定其作为避孕疫苗候选物的有用性。
Sera from patients with known or suspected immunological infertility were used to screen a human testis cDNA library. A total of 59 sera detected 38 unique cDNA inserts of which four were testis specific by Northern blot analyses. One of these is a testis-specific isoform of calpastatin. Five additional clones, although not testis specific, were found to be testis abundant. The number and type of clones identified by these human sera suggests a possible aetiology for immunologic infertility. The testis-specific clones will be further characterized to establish their usefulness as contraceptive vaccine candidates.