C-Reaction Protein Detection in Human Saliva by Nanoplasmonic Color Imaging
C-Reaction Protein Detection in Human Saliva by Nanoplasmonic Color Imaging
复制标题
通过纳米等离子体彩色成像检测人唾液中的 C 反应蛋白
DOI：
10.1166/jbn.2019.2769
复制
发表时间：
2019-08-01
影响因子：
2.9
通讯作者：
Liu, Gang Logan
中科院分区：
文献类型：
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作者：
Hu, Wenjun;Dang, Tang;Liu, Gang Logan
报道了一种基于金 - 二氧化钛 - 金金属 - 绝缘体 - 金属（MIM）等离子体纳米杯阵列中等离子体 - 光子相互作用的无标记高灵敏度成像传感器。该传感器能够检测蛋白质，并在可见光传感方面表现出优异性能。这种器件能够基于彩色成像的红色通道强度变化，非常灵敏地检测到上层介质折射率的增加。与其他传统等离子体传感器相比，我们的器件通过使用普通白光实现透射成像检测，并将仪器要求降至最低。在这项研究中，我们使用该器件检测唾液中的C - 反应蛋白（CRP）水平，CRP水平在不同疾病和病症中被广泛检测以辅助临床决策。强度成像显示CRP浓度（从5到100纳克/毫升）与器件中的相对强度变化之间具有良好的线性响应。能够检测到的CRP最低浓度为5纳克/毫升。此外，当血清中的CRP水平为3.2微克/毫升时，它能够在患者的唾液中实现阳性检测。由于MIM等离子体纳米杯阵列的高性能，所提出的器件在未来利用可见光照明和成像的便携式光学传感方面具有应用前景。
A label-free and highly sensitive imaging sensor based on plasmonic-photonic interaction in a gold-titanium dioxide-gold metal-insulator-metal (MIM) plasmonic nanocup array is reported. The sensor can detect proteins and exhibits superior performance in visible light sensing. This device enables very sensitive detection of an increase in superstrate refractive index based on changes in the red channel intensity of color imaging. Compared to other conventional plasmonic sensors, our device achieves transmission imaging detection by using normal white light and minimizes instrumentation requirement. In this study, we used the device to detect C-reaction protein (CRP) level in saliva, which is widely tested to help make clinical decisions in different diseases and disorders. The intensity imaging showed a good linear response between CRP concentration (from 5 to 100 ng/mL) and relative intensity change in the device. The lowest concentration of CRP that could be detected was 5 ng/mL. Moreover, it could achieve a positive detection in saliva from patients when the CRP level in serum was 3.2 mu g/mL. Owing to the high performance of the MIM plasmonic nanocup array, the proposed device is promising for future portable optical sensing with visible light illumination and imaging.