Morphologic comparison of cervical, thoracic, lumbar intervertebral discs of cynomolgus monkey (Macaca fascicularis)
Morphologic comparison of cervical, thoracic, lumbar intervertebral discs of cynomolgus monkey (Macaca fascicularis)
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DOI：
10.1007/s00586-005-0035-2
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发表时间：
2006-12-01
影响因子：
2.8
通讯作者：
Forriol, Francisco
中科院分区：
文献类型：
--
作者：
Longo, Giuseppe;Ripalda, Purificacion;Forriol, Francisco
目的是分析不同水平椎间盘和终板的形态差异。从 9 只 3 至 4 岁的食蟹猴 (Macaca fasciulousis) 的脊柱中获得了 45 个椎骨运动节段。从每个脊柱中切下五个椎间盘（C5-C6、T3-T4、T9-T10、L2-L3、L4-L5）。在所有组中，收集组织样本并用Masson三色、Safranine-O和van Gieson结缔组织染色对切片进行染色以分析椎间盘。使用特异性抗体进行免疫组织化学检测 I 型和 II 型胶原蛋白。通过组织形态学测量椎间盘高度、最大髓核高度、上下终板高度并计算不同指标，比较同一动物标本之间、同一级别椎间盘之间的差异，最终得出不同级别椎间盘组之间的差异。不同级别的椎间盘之间，终板上环形纤维的锚定没有差异。在纵韧带和邻近它们的环形区域观察到 I 型胶原蛋白的阳性免疫反应。在靠近髓核的纤维环、终板和细胞核中发现了 II 型胶原蛋白免疫反应性。不同水平的椎间盘之间胶原蛋白I和II的定位没有差异。椎间盘的高度沿着脊柱变化。 T3-T4 处测得最小值，尾部增幅大于颅部。最高值在 L2-L3 处测量。颈椎间盘高度是腰椎间盘高度的55%。终板高度沿着脊柱长度增加。下等的EP总是高于上等的。该研究提供了椎间盘的详细结构特征，可能有助于进一步研究椎间盘退变过程。
The aim was to analyze the morphological differences of the intervertebral disc and endplates at different levels. Forty-five vertebral motion segments were obtained from the spine of nine 3 to 4-year-old cynomolgus monkeys (Macaca fascicularis). From every spine, five discs were sectioned (C5-C6, T3-T4, T9-T10, L2-L3, L4-L5). In all the groups, tissue samples were collected and sections were stained with Masson's trichrome, Safranine-O and van Gieson's connective tissue stain to analyze the intervertebral discs. Immunohistochemistry was performed, using specific antibodies to detect collagens I and II. The intervertebral disc height, the maximum nucleus pulposus height, the superior and inferior endplate heights were histomorphometrically measured and different indexes were calculated to compare the differences between specimens of the same animal and between discs of the same level, and finally the differences between groups of discs of different levels. There were no differences existing in annular fibers anchoring on the endplate between discs of different levels. A positive immune reaction for type I collagen was observed in the longitudinal ligaments and in the annular region adjacent to them. Collagen II immune reactivity was found in the annulus close to the nucleus pulposus, in the endplates and in the nucleus. There were no differences between discs of different levels in the collagen I and II localization. The height of the discs varied along the spine. The smallest value was measured in T3-T4, with a larger increase caudally than cranially. The highest value was measured in L2-L3. A cervical disc was 55% the height of a lumbar one. The endplate height increased along the length of the spine. The inferior EP was always higher than the superior. The study provides a detailed structural characterization of the intervertebral disc and may be useful for further investigations on the disc degeneration process.