Isolation and characterization of a novel type of rotavirus species A in sugar gliders (Petaurus breviceps).
Isolation and characterization of a novel type of rotavirus species A in sugar gliders (Petaurus breviceps).
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DOI：
10.1099/jgv.0.000433
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发表时间：
2016-05
期刊：
影响因子：
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通讯作者：
Kota Okadera;M. Abe;N. Ito;Hiromichi Mitake;K. Okada;Kento Nakagawa;Y. Une;H. Tsunemitsu;M. Sugiyama
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文献类型：
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作者：
Kota Okadera;M. Abe;N. Ito;Hiromichi Mitake;K. Okada;Kento Nakagawa;Y. Une;H. Tsunemitsu;M. Sugiyama
为了评估轮状病毒A种(RVA)从外来宠物向其他哺乳动物物种传播的风险，调查了轮状病毒A种在白腹滑鼠(Petaurus Brevicep)体内的流行情况。反转录-半套式聚合酶链式反应(RT-半套式PCR)在44只糖滑翔机中检测到10只RVA。这些病毒被归类为G27P[3]和G27P[36]，其中G27和P[36]是轮状病毒分类工作组指定的新的基因类型。为了对糖用滑翔机RVA进行详细鉴定，分离到一株糖用滑翔机RVA/SugarGlider-TC/JPN/SG385/2012/G27P[36](SG385-TC)。所有的基因都被归类为新的基因类型(G27-P[36]-I19-R10-C10-M9-A20-N11-T13-E17-H12).在SG385-TC和以前报道的哺乳动物菌株之间，NSP4的肠毒素结构域是保守的，这表明糖滑翔机RVA可能会引起哺乳动物的腹泻。事实上，九只乳鼠中有七只口服接种了3.9×104f.f.u。菌株SG385-TC对乳鼠的半数致泻量(DD50)为1.2×104f.f.u。我们的发现表明，糖滑翔物RVA对其他哺乳动物物种具有感染性，并且可能是致病性的。
To estimate the risk of interspecies transmission of rotavirus species A (RVA) from exotic pets to other mammalian species, the prevalence of RVA in sugar gliders (Petaurus breviceps) was investigated. RVAs were detected in 10 of 44 sugar gliders by reverse transcription (RT)-semi-nested PCR. These viruses were classified as G27P[3] and G27P[36] genotypes, with G27 and P[36] being new genotypes as assigned by the Rotavirus Classification Working Group. To characterize sugar glider RVA in detail, one strain, RVA/SugarGlider-tc/JPN/SG385/2012/G27P[36] (SG385-tc), was isolated. All of the genes of the strain were classified as new genotypes (G27-P[36]-I19-R10-C10-M9-A20-N11-T13-E17-H12). The enterotoxin domain in NSP4, which is important for the induction of diarrhoea, was conserved between SG385-tc and previously reported mammalian strains, suggesting the potential of sugar glider RVA to cause diarrhoea in mammalian species. In fact, seven out of nine suckling mice inoculated orally with 3.9 × 104 f.f.u. of strain SG385-tc had diarrhoea and the 50 % diarrhoea-inducing dose (DD50) of strain SG385-tc in suckling mice was 1.2 × 104 f.f.u. Our findings suggest that sugar glider RVA is infective to and possibly pathogenic in other mammalian species.