Characterization of esterase activity from an Acetomicrobium hydrogeniformans enzyme with high structural stability in extreme conditions.
Characterization of esterase activity from an Acetomicrobium hydrogeniformans enzyme with high structural stability in extreme conditions.
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极端条件下具有高结构稳定性的氢化醋微菌酶的酯酶活性表征。
DOI：
10.1007/s00792-018-1038-3
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发表时间：
2018
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影响因子：
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通讯作者：
Kumagai PS
中科院分区：
文献类型：
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作者：
Kumagai PS
耐热和耐有机溶剂的酶的生物技术和工业用途是广泛的，从油藏中存在的微生物群中研究这种酶是一种有前途的方法。在序列数据库中搜索来自此类微生物群的酯酶，我们已经通过计算机识别出来自产氢Acetomicrobium hydrogeniformans的潜在分泌酯酶，命名为AhEst。重组酶在E. colito可用于生物化学和生物物理表征研究。AhEst对短酰基链对硝基苯基酯底物具有水解活性。AhEst活性高，在高达75 °C的温度下稳定。有趣的是，高盐浓度诱导催化活性的显着增加。AhEst在30%浓度的几种有机溶剂中仍保留约50%的活性。同步辐射圆二色性和荧光光谱证实，AhEst显示在极端条件下的温度，盐度和有机溶剂的高结构稳定性。该酶是一种良好的乳化剂，并且能够部分逆转油湿碳酸盐基质的润湿性，使其具有用于提高石油采收率的潜在利益。在AhEst中观察到的所有特性使其成为许多工业应用的有趣候选者，例如需要在高温下具有显着水解活性的应用。
The biotechnological and industrial uses of thermostable and organic solvent-tolerant enzymes are extensive and the investigation of such enzymes from microbiota present in oil reservoirs is a promising approach. Searching sequence databases for esterases from such microbiota, we have identified in silico a potentially secreted esterase fromAcetomicrobium hydrogeniformans, named AhEst. The recombinant enzyme was produced inE. colito be used in biochemical and biophysical characterization studies. AhEst presented hydrolytic activity on short-acyl-chainp-nitrophenyl ester substrates. AhEst activity was high and stable in temperatures up to 75 °C. Interestingly, high salt concentration induced a significant increase of catalytic activity. AhEst still retained ~ 50% of its activity in 30% concentration of several organic solvents. Synchrotron radiation circular dichroism and fluorescence spectroscopies confirmed that AhEst displays high structural stability in extreme conditions of temperature, salinity, and organic solvents. The enzyme is a good emulsifier agent and is able to partially reverse the wettability of an oil-wet carbonate substrate, making it of potential interest for use in enhanced oil recovery. All the traits observed in AhEst make it an interesting candidate for many industrial applications, such as those in which a significant hydrolytic activity at high temperatures is required.