Neuroprotective Effects of Bioavailable Polyphenol-Derived Metabolites against Oxidative Stress-Induced Cytotoxicity in Human Neuroblastoma SH-SY5Y Cells
Neuroprotective Effects of Bioavailable Polyphenol-Derived Metabolites against Oxidative Stress-Induced Cytotoxicity in Human Neuroblastoma SH-SY5Y Cells
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DOI：
10.1021/acs.jafc.6b04538
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发表时间：
2017-02-01
影响因子：
6.1
通讯作者：
Carlos Espin, Juan
中科院分区：
文献类型：
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作者：
Gonzalez-Sarrias, Antonio;Angeles Nunez-Sanchez, Maria;Carlos Espin, Juan
氧化应激与神经退行性疾病的细胞死亡有关。饮食多酚可以发挥健康益处，但它们对神经细胞的直接影响是有争议的，因为大多数酚是在代谢过程中产生的，并不像饮食来源那样到达大脑。在此，我们评估了一组生物可用多酚及其衍生代谢产物在生理相关条件下对抗过氧化氢诱导的人神经母细胞瘤SH-SY5Y细胞凋亡的作用。在所测试的19种代谢物中，3，4-二羟基苯丙酸、3，4-二羟基苯乙酸、没食子酸、鞣花酸和尿脂素通过降低ROS水平来阻止神经元的凋亡，增加红牛的活性，并通过阻止caspase-3的激活来减少氧化应激诱导的SH-SY5Y细胞的凋亡。这表明，含有这些分子的多酚前体的饮食来源，如可可、浆果、核桃和茶，可能是潜在的功能食品，可以减少与神经退行性疾病的发生和进展有关的氧化应激。
Oxidative stress is involved in cell death in neurodegenerative diseases. Dietary polyphenols can exert health benefits, but their direct effects on neuronal cells are debatable because most phenolics are metabolized and do not reach the brain as they occur in the dietary sources. Herein, we evaluate the effects of a panel of bioavailable polyphenols and derived metabolites at physiologically relevant conditions against H2O2-induced apoptosis in human neuroblastoma SH-SY5Y cells. Among the 19 metabolites tested, 3,4-dihydroxyphenylpropionic acid, 3,4-dihydroxyphenylacetic acid, gallic acid, ellagic acid, and urolithins prevented neuronal apoptosis via attenuation of ROS levels, increased RED OX activity, and decreased oxidative stress induced apoptosis by preventing the caspase-3 activation via the mitochondrial apoptotic pathway in SH-SY5Y cells. This suggests that dietary sources containing the polyphenol precursors of these molecules such as cocoa, berries, walnuts, and tea could be potential functional foods to reduce oxidative stress associated with the onset and progress of neurodegenerative diseases.