A neonatal rat model of increased right ventricular afterload by pulmonary artery banding
A neonatal rat model of increased right ventricular afterload by pulmonary artery banding
复制标题
通过肺动脉结扎增加右心室后负荷的新生大鼠模型。
DOI：
10.1016/j.jtcvs.2017.06.016
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发表时间：
2017-11-01
影响因子：
6
通讯作者：
Liu, Jinfen
中科院分区：
文献类型：
--
作者：
Wang, Shoubao;Ye, Lincai;Liu, Jinfen
目的：构建新生大鼠右心室后负荷增加模型，用于研究右心室后负荷增加的先天性心脏病患者右心室的病理生理重塑。方法：出生后6小时内进行手术。水平开胸术是通过解剖肋间肌并分裂胸骨来进行的。然后用 11-0 尼龙线将 PA 绑扎起来。出生后第 7 天 (P7)，超声心动图证实 PA 收缩。通过心导管插入术测量右心室收缩压和舒张压。通过磁共振成像评估 RV 收缩末期容积、舒张末期容积、舒张末期直径和游离壁厚度。通过苏木精和伊红染色评估假手术心脏和 PA 结扎 (PAB) 心脏的组织学变化。 结果：出生后 6 小时内通过收缩新生大鼠的 PA 来增加 RV 后负荷。 P7 时的存活率为 75%。相对于假手术组，PAB 组在 P7 时 PA 收缩区和 RV 收缩压和舒张压的峰值压力梯度、收缩末期容积、舒张末期容积、舒张末期直径和自由壁厚度显着增加 (P < .01)。组织学检查一致显示PAB组右心室游离壁显着肥厚。结论：我们在出生后6小时内通过PAB成功建立了新生儿右心室后负荷增加模型，可用于研究先天性心脏病右室后负荷增加的病理生理变化。
Objective: To construct a neonatal rat model of increased right ventricular (RV) afterload for studying the pathophysiological remodeling of the right ventricle in patients with congenital heart disease with increased RVafterload.Methods: Surgery was performed within 6 hours after birth. Horizontal thoracotomy was performed by dissecting the intercostal muscles and splitting the sternum. The PA was then banded with 11-0 nylon thread. At postnatal day 7 (P7), constriction of PAwas confirmed by echocardiography. The RV systolic and diastolic pressures were measured by cardiac catheterization. The RV end-systolic volume, end-diastolic volume, end-diastolic diameter, and free wall thickness were assessed by magnetic resonance imaging. The histological changes in sham-operated and PA-banding (PAB) hearts were evaluated by hematoxylin and eosin staining.Results: Increased RV afterload was established by constriction of the PA in neonatal rats within 6 hours after birth. The survival rate was 75% at P7. Relative to the sham group, the peak pressure gradient across the PA constriction and RV systolic and diastolic pressures, end-systolic volume, end-diastolic volume, enddiastolic diameter, and free wall thickness were significantly increased in the PAB group at P7 (P < .01). Consistently, histological examination showed that the RV free wall was significantly hypertrophic in the PAB group.Conclusions: We successfully established a neonatal RVafterload increase model through PAB within 6 hours after birth, which can be used to study the pathophysiological changes in congenital heart diseases with increased RV afterload.