Neutrophilic differentiation induced by human upper airway fibroblast-derived granulocyte/macrophage colony-stimulating factor (GM-CSF).
Neutrophilic differentiation induced by human upper airway fibroblast-derived granulocyte/macrophage colony-stimulating factor (GM-CSF).
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由人上呼吸道成纤维细胞衍生的粒细胞/巨噬细胞集落刺激因子（GM-CSF）诱导的中性粒细胞分化。
DOI：
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发表时间：
1991
影响因子：
6.4
通讯作者：
M. Jordana
中科院分区：
文献类型：
--
作者：
C. Vancheri;T. Ohtoshi;G. Cox;A. Xaubet;J. Abrams;J. Gauldie;J. Dolovich;J. Denburg;M. Jordana
我们已经建立了鼻息肉（NP）组织以及正常鼻（NN）粘膜的成纤维细胞的原系，并检查了这些细胞释放类肽信使分子（细胞因子）的能力。我们的研究结果表明，人上呼吸道成纤维细胞在体外释放粒细胞/巨噬细胞集落刺激因子（GM-CSF）、粒细胞-CSF（G-CSF）和巨噬细胞-CSF（M-CSF）。我们还表明，从NP组织来源的成纤维细胞表达的GM-CSF基因在更高的水平，并释放更大量的GM-CSF产品，比NN成纤维细胞。此外，我们还研究了这些成纤维细胞及其条件培养基（CM）诱导人造血祖细胞分化的能力。7天后，这些细胞在RPMI-10%胎牛血清中的培养物含有5 +/- 2.5%（平均值+/- SD）中性粒细胞。相比之下，祖细胞与成纤维细胞的培养导致显著更大的嗜酸性分化（18 +/-4%）。在成纤维细胞-CM中培养诱导了相似程度的分化，并且与来自NN成纤维细胞的CM相比，来自NP成纤维细胞的成纤维细胞-CM引起了更大的分化（17.5 +/-3对12 +/-3%）。用抗人GM-CSF的单克隆中和抗体预孵育成纤维细胞-CM，可完全抑制其诱导的嗜酸性细胞分化。因此，我们的结果表明：（1）人上呼吸道成纤维细胞在体外释放GM-、G-和M-CSF的能力;（2）与NN成纤维细胞相比，来源于NP组织的成纤维细胞表达该基因并以更高的水平释放产物GM-CSF;和（3）来源于成纤维细胞的GM-CSF引起人造血祖细胞的嗜酸性分化。
We have established primary lines of fibroblasts from nasal polyp (NP) tissues as well as from normal nasal (NN) mucosa and have examined the ability of these cells to release hormone-like peptide messenger molecules (cytokines). Our results show that human upper airway fibroblasts release granulocyte/macrophage colony-stimulating factor (GM-CSF), granulocyte-CSF (G-CSF), and macrophage-CSF (M-CSF) in vitro. We also show that fibroblasts derived from NP tissue express the gene for GM-CSF at a higher level, and release the GM-CSF product in greater amounts, than NN fibroblasts. In addition, we have examined the ability of these fibroblasts and their conditioned medium (CM) to induce differentiation of human hemopoietic progenitor cells. After 7 d, cultures of these cells in RPMI-10% fetal bovine serum contained 5 +/- 2.5% (mean +/- SD) neutrophils. In contrast, culture of progenitor cells with fibroblasts resulted in significantly greater neutrophilic differentiation (18 +/- 4%). Culture in fibroblast-CM induced a similar degree of differentiation, and fibroblast-CM from NP fibroblasts elicited greater differentiation compared to CM from NN fibroblasts (17.5 +/- 3 versus 12 +/- 3%). The neutrophilic differentiation induced by fibroblast-CM can be fully inhibited by preincubating this CM with a monoclonal neutralizing antibody to human GM-CSF. Thus, our results demonstrate: (1) the ability of human upper airway fibroblasts to release GM-, G-, and M-CSF in vitro; (2) that fibroblasts derived from NP tissues express the gene and release the product GM-CSF at greater levels compared to NN fibroblasts; and (3) that fibroblast-derived GM-CSF causes neutrophilic differentiation of human hemopoietic progenitors.
重组人肿瘤坏死因子α和β在体外刺激成纤维细胞产生造血生长因子。
DOI：
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发表时间：
1988
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Zucali,JR;Broxmeyer,HE;Gross,MA;Dinarello,CA
通讯作者：
Dinarello,CA
集落刺激因子的分子生物学。
DOI：
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发表时间：
1987
期刊：
Blood cells
影响因子：
--
作者：
Kaushansky,K
通讯作者：
Kaushansky,K
影响因子：
20.3
作者：
Wang,JM;Chen,ZG;Colella,S;Bonilla,MA;Welte,K;Bordignon,C;Mantovani,A
通讯作者：
Mantovani,A