Wide-field Raman imaging of dental lesions.
Wide-field Raman imaging of dental lesions.
复制标题
牙齿病变的宽视场拉曼成像。
DOI：
10.1039/c4an00164h
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Lang,Lisa
中科院分区：
文献类型：
--
作者：
Yang,Shan;Li,Bolan;Akkus,Anna;Akkus,Ozan;Lang,Lisa
龋齿的早期检测仍然是牙科医学的一大挑战。由于拉曼光谱对牙釉质中最丰富的拉曼活性矿物晶体的数量敏感，因此可以成功地应用于检测龋病。有效的诊断需要通过拉曼成像对牙齿表面进行全面检查。由于数据采集时间较长，点扫描拉曼制图与临床无关（可行）。本研究基于高灵敏度二维CCD相机组装了一套宽视场拉曼成像系统，用于对病变牙齿的矿化状态进行成像。宽视场图像显示一些病变低矿化，其他病变高矿化。宽视场拉曼成像结果与点扫描拉曼成像结果一致。因此，可以通过拉曼成像的矿物质含量来区分健全的牙釉质和病变。总之，在临床上，宽视场拉曼成像是一种潜在的有用的牙齿病变可视化工具。
Detection of dental caries at the onset remains as a great challenge in dentistry. Raman spectroscopy could be successfully applied towards detecting caries since it is sensitive to the amount of Raman active mineral crystals, the most abundant component of enamel. Effective diagnosis requires full examination of a tooth surface via Raman mapping. Point-scan Raman mapping is not clinically relevant (feasible) due to lengthy data acquisition time. In this work, a wide-field Raman imaging system was assembled based on a high-sensitivity 2D CCD camera for imaging the mineralization status of teeth with lesions. Wide-field images indicated some lesions to be hypomineralized and others to be hypermineralized. The observations of wide-field Raman imaging were in agreement with point-scan Raman mapping. Therefore, sound enamel and lesions can be discriminated by Raman imaging of the mineral content. In conclusion, wide-field Raman imaging is a potentially useful tool for visualization of dental lesions in the clinic.