The Complex Ultrastructure of the Endolysosomal System
The Complex Ultrastructure of the Endolysosomal System
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DOI：
10.1101/cshperspect.a016857
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发表时间：
2014-10-01
影响因子：
7.2
通讯作者：
Raposo, Graca
中科院分区：
文献类型：
--
作者：
Klumperman, Judith;Raposo, Graca
活细胞成像揭示了内溶酶体系统作为一个复杂的和高度动态的网络相互作用的隔间。不同类型的内体通过动力学、分子和形态学标准来辨别。虽然这些标准或其组合都不能捕获内溶酶体系统的全部复杂性，但它们对于实验目的非常有用。一些膜结构域的专门化和特定的形态特征只能通过制备电子显微镜（EM）后的超微结构分析来观察。免疫EM允许通过其分子组成进一步区分看似相同的隔室。在这篇综述中，我们提供了一个概述的超微结构特征和膜组织的内体隔间，沿着与他们的组织机构。
Live-cell imaging reveals the endolysosomal system as a complex and highly dynamic network of interacting compartments. Distinct types of endosomes are discerned by kinetic, molecular, and morphological criteria. Although none of these criteria, or combinations thereof, can capture the full complexity of the endolysosomal system, they are extremely useful for experimental purposes. Some membrane domain specializations and specific morphological characteristics can only be seen by ultrastructural analysis after preparation for electron microscopy (EM). Immuno-EM allows a further discrimination of seemingly identical compartments by their molecular makeup. In this review we provide an overview of the ultrastructural characteristics and membrane organization of endosomal compartments, along with their organizing machineries.