A step change in the transfer of interspecific variation into wheat from Amblyopyrum muticum.
A step change in the transfer of interspecific variation into wheat from Amblyopyrum muticum.
复制标题
种间变异转移到小麦中的步骤变化。
DOI：
10.1111/pbi.12606
复制
发表时间：
2017-02
影响因子：
13.8
通讯作者：
King IP
中科院分区：
文献类型：
--
作者：
King J;Grewal S;Yang CY;Hubbart S;Scholefield D;Ashling S;Edwards KJ;Allen AM;Burridge A;Bloor C;Davassi A;da Silva GJ;Chalmers K;King IP
尽管取得了一些显著的成功，但只有一小部分野生近缘种的遗传变异被用来培育上级小麦品种。这是由于缺乏合适的高通量技术来检测小麦/野生相对基因渗入的直接结果。在这里，我们报告使用一个新的SNP阵列检测小麦/野生的相对基因渗入来自种间六倍体小麦/Ambylopyrum muticum F1杂种回交后代。该阵列使得能够检测和表征218个全基因组小麦/Am. muticum基因渗入，与该领域的先前工作相比，这是基因渗入的产生和检测的显著步骤变化。此外，检测到的基因渗入频率足够高，能够构建Am. muticum基因组的7个连锁群，从而能够确定野生近缘种和六倍体小麦之间的同线关系。讨论了将野败枝菌遗传变异导入小麦对培育上级品种的重要性。
Despite some notable successes, only a fraction of the genetic variation available in wild relatives has been utilized to produce superior wheat varieties. This is as a direct result of the lack of availability of suitable high‐throughput technologies to detect wheat/wild relative introgressions when they occur. Here, we report on the use of a new SNP array to detect wheat/wild relative introgressions in backcross progenies derived from interspecific hexaploid wheat/Ambylopyrum muticum F1 hybrids. The array enabled the detection and characterization of 218 genomewide wheat/Am. muticum introgressions, that is a significant step change in the generation and detection of introgressions compared to previous work in the field. Furthermore, the frequency of introgressions detected was sufficiently high to enable the construction of seven linkage groups of the Am. muticum genome, thus enabling the syntenic relationship between the wild relative and hexaploid wheat to be determined. The importance of the genetic variation from Am. muticum introduced into wheat for the development of superior varieties is discussed.