A combined immunofluorescence and fluorescent in situ hybridization assay for single cell analyses of dental plaque microorganisms
A combined immunofluorescence and fluorescent in situ hybridization assay for single cell analyses of dental plaque microorganisms
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DOI：
10.1016/j.mimet.2006.12.012
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发表时间：
2007-05-01
影响因子：
2.2
通讯作者：
Luethi-Schaller, Helga
中科院分区：
文献类型：
--
作者：
Gmuer, Rudolf;Luethi-Schaller, Helga
IF-FISH方案被开发用于表征复杂牙菌斑样品内的离散细胞群。优化和缩短3步IF程序并在所有IF试剂中包括RNA酶抑制剂使得能够组合两种技术，从而能够同时检测和计数具有不同16 S rRNA序列和/或感兴趣的表面标志物的细菌种群。(C)2007 Elsevier B. V.保留所有权利。
A IF-FISH protocol was developed to characterize discrete cell populations within complex dental plaque samples. Optimizing and shortening the 3-step IF procedure and including RNase inhibitor in all IF reagents enabled the combination of both techniques and thus the simultaneous detection and enumeration of bacteria] populations with distinct 16S rRNA sequences and/or surface markers of interest. (C) 2007 Elsevier B.V. All rights reserved.