Production of ethanol from mannitol by the yeast strain Saccharomyces paradoxus NBRC 0259
Production of ethanol from mannitol by the yeast strain Saccharomyces paradoxus NBRC 0259
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DOI：
10.1016/j.jbiosc.2013.03.018
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发表时间：
2013-09-01
影响因子：
2.8
通讯作者：
Murata, Kousaku
中科院分区：
文献类型：
--
作者：
Ota, Anri;Kawai, Shigeyuki;Murata, Kousaku
甲烷是一种很有前景的海洋大型藻类碳源。然而，从甘露醇生产乙醇的生物是有限的;迄今为止，只有酵母安哥拉毕赤酵母和细菌大肠杆菌1（011）据报道具有这种能力。在这项研究中，我们寻找了一个酵母菌株具有较高的能力，从甘露醇生产乙醇，并选择了奇异酵母NBRC 0259的能力，从甘露醇生产乙醇。该菌株在含甘露醇的培养基中培养3天后，这种能力得到增强，将增强的菌株重新命名为S。paradoxus NBRC 0259-3.我们比较了菌株NBRC 0259-3在几种条件下从甘露醇和葡萄糖生产乙醇的能力，并与安哥拉假单胞菌和E. coliKO 11。paradoxus NBRC 0259-3菌株是最适合从甘露醇生产乙醇的酵母菌株。(C)2013年，生物技术学会，日本。All rights reserved.
Mannitol is a promising marine macroalgal carbon source. However, organisms that produce ethanol from mannitol are limited; to date, only the yeast Pichia angophorae and the bacterium Escherichia coli 1(011 have been reported to possess this capacity. In this study, we searched a yeast strain with a high capacity to produce ethanol from mannitol and selected Saccharomyces paradoxus NBRC 0259 for its ability to produce ethanol from mannitol. This ability was enhanced after a 3-day cultivation of this strain in medium containing mannitol; the enhanced strain was renamed S. paradoxus NBRC 0259-3. We compared the ability of strain NBRC 0259-3 to produce ethanol from mannitol and glucose, under several conditions, with those of P. angophorae and E. coli KO11 As a result, we concluded that S. paradoxus NBRC 0259-3 strain is the most suitable yeast strain for the production of ethanol from mannitol. (C) 2013, The Society for Biotechnology, Japan. All rights reserved.