An isolated tumor perfusion model in mice.
An isolated tumor perfusion model in mice.
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DOI：
10.1038/nprot.2012.030
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发表时间：
2012-03-22
期刊：
影响因子：
14.8
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中科院分区：
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间质细胞在肿瘤微环境中的作用已被广泛描述。我们和其他人已经证明，基质细胞可能参与了转移级联的几个步骤。该方案描述了一种孤立的肿瘤灌注模型，该模型能够研究癌症和间质细胞脱落。它还可以用来研究干扰肿瘤细胞或碎片、循环(干细胞)细胞或生物标记物脱落的治疗效果。原发肿瘤生长在基质细胞无处不在表达GFP的微环境中。肿瘤可以在原位植入，也可以在异位植入。因此，所有肿瘤相关的间质细胞都表达GFP。这项技术可用于检测和研究小鼠循环内肿瘤碎片中基质细胞的作用。使用这个模型研究基质细胞在循环肿瘤碎片中的作用可能需要2-10周，这取决于原发肿瘤的生长速度。
The role of stromal cells in the tumor microenvironment has been extensively characterized. We and others have shown that stromal cells may participate in several steps of the metastatic cascade. This protocol describes an isolated tumor perfusion model that enables studies of cancer and stromal cell shedding. It could also be used to study the effects of therapies interfering with the shedding of tumor cells or fragments, circulating (stem) cells or biomarkers. Primary tumors are grown in a microenvironment in which stromal cells express GFP ubiquitously. Tumors are implanted orthotopically or can be implanted ectopically. As a result, all tumor-associated stromal cells express GFP. This technique can be used to detect and study the role of stromal cells in tumor fragments within the circulation in mice. Studying the role of stromal cells in circulating tumor fragments using this model may take 2–10 weeks, depending on the growth rate of the primary tumor.