Subgingival microflora of periodontal patients on tetracycline therapy.
Subgingival microflora of periodontal patients on tetracycline therapy.
复制标题
接受四环素治疗的牙周患者龈下微生物群落。
DOI：
10.1111/j.1600-051x.1979.tb01923.x
复制
发表时间：
1979
影响因子：
6.7
通讯作者：
J. Jorgensen
中科院分区：
文献类型：
--
作者：
B. Williams;S. Osterberg;J. Jorgensen
龈下菌斑样本收集自接受常规牙周治疗后两种不同四环素治疗方案的牙周病患者。4例患者口服1000 mg/天，持续2周，9例患者接受1000 mg/天，持续1周，随后接受250 mg/天，持续更长时间。后一种方案是类似的，因此通常用于慢性皮肤病，痤疮。菌斑分离株的分类学表征表明，从两个处理组培养的主要微生物是链球菌属和分支丝状革兰氏阳性杆菌、放线菌属和罗氏菌属。在从服用250 mg/天的患者中分离的植物群中观察到更大的微生物复杂性。后一组窝藏许多挑剔的革兰氏阴性微生物目前牵连在牙周病的病因。通过在含有抗生素两倍稀释液的琼脂培养基上复制平板培养，检测所有分离的细菌菌株对四环素的耐药性。链球菌属、韦荣氏球菌属和奈瑟氏球菌属被发现对四环素具有一致的耐药性，最低抑菌浓度高达128微克/毫升。在放线菌中，抗生素抗性的选择仅在一个物种中常见，A。溶牙菌然而，从一名患者的龈下植物群中分离出的54%的粘性放线菌菌株确实表现出中等耐药性水平，在浓度高达8微克/毫升时生长。从低剂量组分离的多种革兰氏阴性杆菌显示四环素耐药。虽然研究的患者数量很少，但对主要龈下细菌的广泛微生物分析表明，在1000 mg/天的2周治疗期间达到了四环素治疗的目标。
Samples of subgingival plaque were collected from periodontal patients receiving two different tetracycline treatment regimens following conventional periodontal therapy. Four patients had received an oral dose of 1000 mg/day for 2 weeks and nine had received 1000 mg/day for 1 week followed by 250 mg/day for extended time periods. The latter regimen is similar so that commonly prescribed for the chronic skin disease, acne vulgaris. Taxonomic characterization of plaque isolates indicated that the predominant organisms cultured from both treatment groups were species of Streptococcus and the branching, filamentous Gram-positive rods, Actinomyces and Rothia. A much greater microbial complexity was observed among the flora isolated from patients taking 250 mg/day. The latter group harbored many of the fastidious Gram-negative organisms presently implicated in the etiology of periodontal disease. All bacterial strains isolated were tested for resistance to tetracycline by replica-plating cultures on an agar medium containing two-fold dilutions of the antibiotic. Streptococcus, Veillonella and Neisseria were the bacterial genera found to be consistently resistant to tetracycline, with minimum inhibitory concentrations as high as 128 micrograms/ml. Among the Actinomyces, selection for antibiotic resistance was common only within a single species, A. odontolyticus. However, A viscosus strains isolated at 54% of the subgingival flora from one patient did demonstrate an intermediate resistance level, growing on concentrations up to 8 micrograms/ml. Tetracycline resistance was shown by a variety of Gram-negative rods isolated from the low dosage group. Although a minimum number of patients were studied, extensive microbial analyses of the predominating subgingival bacteria indicated that the goal of tetracycline therapy was achieved during the 2-week therapy period at 1000 mg/day.