Detection of Penicillin via Surface Plasmon Resonance Biosensor
Detection of Penicillin via Surface Plasmon Resonance Biosensor
复制标题
通过表面等离子共振生物传感器检测青霉素
DOI：
--
复制
发表时间：
2012-09
期刊：
影响因子：
--
通讯作者：
MU Ying*
中科院分区：
文献类型：
--
作者：
ZHANG Ying, JIN Wei, YANG Meng-chao;ZHANG Ti-qiang, ZHOU Chao, XIE Fei,;SONG Qi, REN Hao, JIN Qin-han;MU Ying*
A method of using Au colloid to capture the decomposed product of penicillin, penicillamine, on a surface plasmon resonance(SPR) biosensor for the quantitative determination of penicillin was developed. Based on the decomposition of penicillin to generate penicillamine and penilloaldehyde, a high sensitive biosensor for detecting penicillin was also developed. In our experiment, it was penicillamine rather than penicillin that has been measured. This is because penicillamine contains a functional group that makes it self-assembling on Au colloid to increase the molecular weight so as to improve the surface plasmon resonance signal. On a UV-Vis spectrophotometer, a high concentration of penicilliamine-Au complex was determined, indicating that penicillamine was already well combined with Au colloid. The method, using the combination of Au colloid with penicillamine, proved to detect penicillin.
DOI：
10.1016/0300-9629(89)90683-x
发表时间：
1988-11
期刊：
The Yale Journal of Biology and Medicine
影响因子：
--
作者：
G. Mckhann
通讯作者：
G. Mckhann