Identifying direct targets of transcription factor Rfx2 that coordinate ciliogenesis and cell movement.
Identifying direct targets of transcription factor Rfx2 that coordinate ciliogenesis and cell movement.
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DOI：
10.1016/j.gdata.2014.06.015
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发表时间：
2014-12-01
期刊：
影响因子：
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通讯作者：
Marcotte, Edward M
中科院分区：
文献类型：
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作者：
Kwon, Taejoon;Chung, Mei-I;Gupta, Rakhi;Baker, Julie C;Wallingford, John B;Marcotte, Edward M
最近，使用青蛙非洲爪蟾作为模型系统，我们发现转录因子 Rfx2 协调许多参与多纤毛细胞纤毛发生和细胞运动的基因（Chung 等，2014）。据我们所知，这是第一篇利用正在进行的非洲爪蟾基因组计划的基因组资源的论文，包括基因组序列和临时基因注释。对于对非洲爪蟾研究中基因组学和系统生物学方法的应用感兴趣的研究人员，我们在此提供有关我们的数据集（NCBI GEO 登录号 GSE50593）的更多详细信息，并描述我们如何分析 RNA-seq 和 ChIP-seq 数据以识别 Rfx2 的直接靶点。
Recently, using the frog Xenopus laevis as a model system, we showed that the transcription factor Rfx2 coordinates many genes involved in ciliogenesis and cell movement in multiciliated cells (Chung et al., 2014). To our knowledge, it was the first paper to utilize the genomic resources, including genome sequences and interim gene annotations, from the ongoing X. laevis genome project. For researchers who are interested in the application of genomics and systems biology approaches in Xenopus studies, here we provide additional details about our dataset (NCBI GEO accession number GSE50593) and describe how we analyzed RNA-seq and ChIP-seq data to identify direct targets of Rfx2.