Comparison of chlorophyll-a concentrations measured by fluorometric HPLC and spectrophotometry methods in highly eutrophic shallow Lake Kasumigaura: With 5 figures in the text
Comparison of chlorophyll-a concentrations measured by fluorometric HPLC and spectrophotometry methods in highly eutrophic shallow Lake Kasumigaura: With 5 figures in the text
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高度富营养化浅层霞浦湖中荧光高效液相色谱法和分光光度法测量叶绿素-a浓度的比较：文中附有5个数字
DOI：
10.1080/03680770.1987.11899747
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
N. Takamura
中科院分区：
文献类型：
--
作者：
A. Otsuki;N. Takamura
虽然已知用三色方程（三色法，STRJCKLAND & PARSONS 1972）进行叶绿素色素的光谱估算存在相当大的误差（如MARLiR等，1980），但由于缺乏简单可靠的方法，已被广泛采用。这种三色法的缺点是浮游植物样品的90%丙酮或100%甲醇提取物含有各种副色素和叶绿素色素降解产物，它们的吸收光谱与叶绿素色素的吸收光谱重叠。近年来，随着化学键合固定相和高压泵的发展，高效液相色谱（HPLC）已成为最有效、最可靠的分离技术之一，并广泛接受使用反相柱测定叶绿素色素（例如，MANTOURA & LLEWELLYN 1983，BIDIGARE等人1985）。
Although spectrometric estimation of chlorophyll pigments using trichromatic equations (trichromatic method, STRJCKLAND & PARSONS 1972) has been known to be involved in considerable errors (eg MARliR et al. 1980), it has been widely done because of laek of the simple and reliable method. A shortcoming of this trichromatic method is that 90% acetone or 100% methanol extracts from phytoplankton samples contain various accessory pigments and degradation products of chlorophyll pigments which have the absorption spectra overlapping with those of chlorophyll pigments. Thus the best way would be to determine each chlorophyll pigment after chromatographic separation.In recent years with the development of chemically bonded stationary phase and high pressure pumps, high performance liquid chromatography (HPLC) has become one of the most efficient and reliable separation techniques, and is receiving a wide acceptance for the determination of chlorophyll pigments using reverse phase column (eg MANTOURA & LLEWELLYN 1983, BIDIGARE et al. 1985).