Reduced motion artifacts and speed improvements in enhanced line-scanning fiber bundle endomicroscopy.
Reduced motion artifacts and speed improvements in enhanced line-scanning fiber bundle endomicroscopy.
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DOI：
10.1117/1.jbo.26.5.056501
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发表时间：
2021-05
影响因子：
3.5
通讯作者：
Hughes M
中科院分区：
文献类型：
--
作者：
Thrapp A;Hughes M
意义：共聚焦激光扫描可在纤维束显微内镜中进行光学切片，但限制了帧率。为了能够更好地探索组织形态，将顺序采集的帧拼接成马赛克是有用的。然而，低帧速率限制了最大探头平移速度。线扫描（LS）共焦显微内镜提供了更高的帧速率，但残留的焦点外的光退化的图像。基于减法的方法可以以引入运动伪影为代价来抑制这种残留。目的：为了生成具有改进的光学切片的高帧率显微内镜图像，我们开发了一种高速减法方法，该方法仅需要采集单个相机帧。方法：CMOS相机的滚动快门充当基于减法的切片增强所需的对准和偏移检测器狭缝。束的两个图像形成在相机的不同区域上，允许同时获取两个图像。结果如下：我们确认改进的光学切片相比，传统的LS，特别是远离焦点，并表明运动伪影不引入。我们展示了高速马赛克的帧速率高达240赫兹。结论：使用新的基于减法的方法的光学切片图像的高速采集导致在高帧速率下改进的拼接。
Significance: Confocal laser scanning enables optical sectioning in fiber bundle endomicroscopy but limits the frame rate. To be able to better explore tissue morphology, it is useful to stitch sequentially acquired frames into a mosaic. However, low frame rates limit the maximum probe translation speed. Line-scanning (LS) confocal endomicroscopy provides higher frame rates, but residual out-of-focus light degrades images. Subtraction-based approaches can suppress this residue at the expense of introducing motion artifacts. Aim: To generate high-frame-rate endomicroscopy images with improved optical sectioning, we develop a high-speed subtraction method that only requires the acquisition of a single camera frame. Approach: The rolling shutter of a CMOS camera acts as both the aligned and offset detector slits required for subtraction-based sectioning enhancement. Two images of the bundle are formed on different regions of the camera, allowing both images to be acquired simultaneously. Results: We confirm improved optical sectioning compared to conventional LS, particularly far from focus, and show that motion artifacts are not introduced. We demonstrate high-speed mosaicing at frame rates of up to 240 Hz. Conclusion: High-speed acquisition of optically sectioned images using the new subtraction based-approach leads to improved mosaicing at high frame rates.