Barcoded Nucleotides
Barcoded Nucleotides
复制标题
DOI：
10.1002/anie.201105717
复制
发表时间：
2012-01-01
影响因子：
16.6
通讯作者：
Marx, Andreas
中科院分区：
文献类型：
--
作者：
Baccaro, Anna;Steck, Anna-Lena;Marx, Andreas
DNA作为一种信息存储系统，由于四种天然核苷酸的不同排列，既简单又复杂。[1]DNA聚合酶的DNA合成是耐人寻味的，因为这些酶能够通过识别DNA模板并选择相应的核苷酸来催化引物链的延长。[1B，2]这一特征可以被利用来通过用修饰的类似物取代天然底物来使四个碱基的密码多样化。[3]配备各种标记基团(如染料、标签或自旋标记[4])的核苷酸类似物可用于DNA聚合酶催化的反应，以扩大DNA的应用范围(如测序、测序、标记或自旋标记结构表征和固定化[4d，5])。嵌入在标记组中的“信息”允许从结果信号的评估中得出结论。通过嵌入显示条形码属性的标记，将导致信息的显著增加。通常，条形码标签没有描述性数据，但它由一系列符号组成，这些符号表示存储的信息(该术语以前曾用于DNA的其他上下文中)。[6]为了普遍采用条形码，条形码应该简单、容易粘贴，并允许可靠地分配存储的信息。寡核苷酸(ODN)在很大程度上符合条形码标签的要求，因为它们具有简单的代码，并且可以通过
DNA as an information storage system is simple and at the same time complex owing to the various different arrangements of the four natural nucleotides.[1] The DNA synthesis by DNA polymerases is intriguing, since these enzymes are able to catalyze the elongation of the primer strand by recognizing the DNA template and selecting the corresponding nucleotide.[1b, 2] This feature can be exploited to diversify the four-base-code by substitution of the natural substrates with modified analogues.[3] Nucleotide analogues equipped with various marker groups (eg dyes, tags, or spin labels [4]) can be employed in DNA polymerase catalyzed reactions to increase the application scope of DNA (eg sequencing, structural characterization, and immobilization [4d, 5]). The “information” embedded in the marker groups allow conclusions to be drawn from the evaluation of the resulting signals. A significant gain in information would result by embedding a marker that exhibits the properties of a barcode. Typically, the barcode label bears no descriptive data but it consists of a series of signs which code for the deposited information (the term was used in other contexts with DNA before).[6] For universal adoption the barcode should be simple, affixed easily, and allow a reliable assignment of the deposited information. Oligodeoxynucleotides (ODNs) meet the requirements of a barcode label to a great extent, since they have a simple code and can be distinguished by