Single nucleotide polymorphism (SNP) genotyping as basis for developing a PCR-based marker highly diagnostic for potato varieties with high resistance to Globodera pallida pathotype Pa2/3
Single nucleotide polymorphism (SNP) genotyping as basis for developing a PCR-based marker highly diagnostic for potato varieties with high resistance to Globodera pallida pathotype Pa2/3
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DOI：
10.1007/s11032-006-9026-1
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发表时间：
2006-11-01
影响因子：
3.1
通讯作者：
Gebhardt, Christiane
中科院分区：
文献类型：
--
作者：
Sattarzadeh, Amirali;Achenbach, Ute;Gebhardt, Christiane
苍白球皮线虫是马铃薯的一种寄生性根胞线虫，可引起马铃薯产量和品质的下降。田间种群G.含有Pa 2和Pa 3（Pa 2/3）致病型混合物的pallida是目前中欧马铃薯栽培中最相关的。抗病基因。pallida已经从野生的、结有块茎的茄属物种S. spegazzinii和S. vernei。在育种工作中，对棉红霉病抗性的表型鉴定一直是筛选抗性基因型的障碍。pallida耗时、昂贵，而且往往含糊不清。以DNA为基础的抗G. pallida将促进抗性品种的开发。利用四倍体分离技术构建了一个对苍白球线虫具有数量抗性的四倍体杂种F1代SR-Gpa家系，并对其进行了抗性鉴定。pallida population 'Chavornay'.选择了由30个高抗个体和30个易感个体组成的两个亚群，并对标记10条马铃薯染色体上12个基因组区域的96个单核苷酸多态性（SNP）标记进行基因型分析。发现7个SNPs与线虫抗性显著相关，它们都位于马铃薯V染色体上的抗性热点内。从SR-Gpa家族中观察到的SNP模式推导出这7个SNPs的单倍型模型。开发了PCR测定HC'，其特异性地检测与高水平的线虫抗性相关的SNP单倍型c。HC标记只发现在加入的S. vernei。利用HC标记筛选了34个抗G.结果表明，HC标记对白叶枯病菌Pa 2和/或Pa 3的高水平抗性具有较高的诊断价值。苍白病型Pa 2/Pa 3。
Globodera pallida is a parasitic root cyst nematode of potato, which causes reduction of crop yield and quality in infested fields. Field populations of G. pallida containing mixtures of pathotypes Pa2 and Pa3 (Pa2/3) are currently most relevant for potato cultivation in middle Europe. Genes for resistance to G. pallida have been introgressed into the cultivated potato gene pool from the wild, tuber bearing Solanum species S. spegazzinii and S. vernei. Selection of resistant genotypes in breeding programs is hampered by the fact that the phenotypic evaluation of resistance to G. pallida is time consuming, costly and often ambiguous. DNA-based markers diagnostic for resistance to G. pallida would facilitate the development of resistant varieties. A tetraploid F-1 hybrid family SR-Gpa segregating for quantitative resistance to G.pallida was developed and evaluated for resistance to G. pallida population 'Chavornay'. Two subpopulations of 30 highly resistant and 30 susceptible individuals were selected and genotyped for 96 single nucleotide polymorphism (SNP) markers tagging 12 genomic regions on 10 potato chromosomes. Seven SNPs were found significantly linked to the nematode resistance, which were all located within a resistance hotspot' on potato chromosome V. A haplotype model for these seven SNPs was deduced from the SNP patterns observed in the SR-Gpa family. A PCR assay HC' was developed, which specifically detected the SNP haplotype c that was linked with high levels of nematode resistance. The HC marker was only found in accessions of S.vernei. Screening with the HC marker 34 potato varieties resistant to G. pallida pathotypes Pa2 and/or Pa3 and 22 susceptible varieties demonstrated that the HC marker was highly diagnostic for presence of high levels of resistance to G. pallida pathotype Pa2/Pa3.