Changes of Cell Wall Degrading Enzymes in Potato Tuber Tissue Slices Infected by Fusarium sulphureum
Changes of Cell Wall Degrading Enzymes in Potato Tuber Tissue Slices Infected by Fusarium sulphureum
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
Yang Zhimin;Bi Yang;Li Yongcai;Kou Zong-hong;Bao GaiHong;Liu Chengkun;Wang Yi;Wang Di
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文献类型：
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作者：
Yang Zhimin;Bi Yang;Li Yongcai;Kou Zong-hong;Bao GaiHong;Liu Chengkun;Wang Yi;Wang Di
【Objective】The dynamic changes of main cell wall degrading enzymes(CWDEs) activities in potato tuber tissue slices infected by Fusarium sulphureum were investigated.【Method】Major CWDEs activities of potato tuber slices tissue(Longshu No.3) inoculated with F.sulphureum were detected and comparatively analyzed during different sampling period.【Result】 A series of CWDEs including polygalacturonase(PG),polymethyl-galacturonase(PMG),carboxymethyl cellulose(Cx),β-glucosidase,polygalacturonic acid trans-eliminase(PGTE),pectin methyl-trans-eliminase(PMTE),pectin methylesterase(PE) and pectate lyase were detected in infected tissue.Whereas PG,PMG,Cx,and β-glucosidase showed higher activities as compared with others.The activity of PMG and Cx kept higher during early infection period(1-3 days after infection),but higher PG activity appeared during later infection period(4-6 days after inoculation),but β-glucosidase showed a rising trend during the enzyme dynamics analysis.【Conclusion】 These findings suggested that CWDEs were the primary pathogenic factors,and different CWDEs might take effect at different infecting periods.
