Human fetal membrane contribution to the prevention of parturition: uterotonin degradation.
Human fetal membrane contribution to the prevention of parturition: uterotonin degradation.
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DOI：
10.1210/jcem.78.2.8106636
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发表时间：
1994-02
期刊：
影响因子：
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通讯作者：
A M Germain;J. Smith;M. Casey;P. MacDonald
中科院分区：
文献类型：
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作者：
A M Germain;J. Smith;M. Casey;P. MacDonald
本研究旨在评价人胎儿膜-壁蜕膜，特别是绒毛膜层降解羊膜中产生的、羊水中存在的或两者兼有的子宫收缩素的潜在能力。最常被评价为子宫肌层收缩剂的四种子宫收缩素可能参与人类分娩的启动，它们是异莲心素、催产素、内皮素-1和血小板活化因子。我们评估了mRNA水平和脑啡肽酶（质膜内肽酶，降解内皮素）和前列腺素脱氢酶（PGDH）在人胎膜，即羊膜和绒毛膜叶，并在壁蜕膜的比活性（SA）。还测定了这些组织中催产素酶（使催产素失活）的SA。在所有研究的妊娠中，来自所有解剖部位（单胎和双胎-双胎胎盘）的绒毛膜层中脑啡肽酶的SA（平均值+/- SEM，95 +/- 7.9 ng/min.mg蛋白; n = 28）与人胎儿肾脏中的SA（89.5 +/- 2.8; n = 6）相似。肾组织被认为是脑啡肽酶最丰富的来源之一。羊膜（18.3 +/- 2.3 nmol/min.mg protein; n = 29）和壁蜕膜（31.8 +/- 6.7; n = 20）中脑啡肽酶的SA也很高，但显著低于绒毛膜叶。单胎妊娠绒毛膜层中脑啡肽酶mRNA水平较高，脑啡肽酶SA也较高（111.9 +/- 10.6 nmol/min.mg蛋白; n = 17）。在5例双胎胎盘的配对绒毛膜组织中，反射绒毛膜（74 ± 12.8，与单胎比较P < 0.06）和融合绒毛膜（64.8 ± 6.5，与单胎比较P < 0.001）的脑啡肽酶SA相似。反射绒毛膜叶中PGDH的SA（46.3 +/- 6.9 nmol/min.mg蛋白; n = 19）显著大于蜕膜中的SA（16 +/- 5.5; n = 15）。PGDH mRNA水平与PGDH酶SA水平呈显著正相关。双胎胎盘融合绒毛膜层PGDH的SA值（14.9 ± 7.3; n = 4）明显低于同胎胎盘反射绒毛膜层PGDH的SA值（70.5 ± 14.7; n = 4）。通过北方分析，在羊膜组织中未检测到PGDH mRNA（n = 5），并且在羊膜中PGDH的SA（< 1.2 +/-1.0; n = 6）未检测到或接近测定检测的下限。（400字处截断摘要）
This investigation was conducted to evaluate the potential capacity of the human fetal membranes-decidua parietalis, and in particular the chorion laeve, to degrade uterotonins that are produced in amnion, are present in amniotic fluid, or both. The four uterotonins that have been evaluated most frequently as myometrial contractants potentially involved in the initiation of human parturition are prostaglandins, oxytocin, endothelin-1, and platelet-activating factor. We assessed the levels of mRNA and the specific activities (SAs) of enkephalinase (the plasma membrane endopeptidase that degrades endothelins) and prostaglandin dehydrogenase (PGDH) in human fetal membranes, i.e. amnion and chorion leave, and in decidua parietalis. The SA of oxytocinase (which inactivates oxytocin) in these tissues also was determined. The SA of enkephalinase in chorion laeve from all anatomical sites (singleton and diamnionic-dichorionic twin placentae) in all pregnancies studied (mean +/- SEM, 95 +/- 7.9 ng/min.mg protein; n = 28) is similar to that in human fetal kidney (89.5 +/- 2.8; n = 6). Kidney tissue is believed to be one of the richest sources of enkephalinase. The SAs of enkephalinase in amnion (18.3 +/- 2.3 nmol/min.mg protein; n = 29) and in decidua parietalis (31.8 +/- 6.7; n = 20) also were high, but significantly less than that in chorion leave. The level of enkephalinase mRNA in chorion laeve in singleton pregnancies is high, as is the SA of enkephalinase (111.9 +/- 10.6 nmol/min.mg protein; n = 17). In paired chorion laeve tissues from five diamnionic-dichorionic twin placentae, the SAs of enkephalinase in reflected chorion laeve (74 +/- 12.8; P < 0.06 compared with singletons) and fused chorion laeve (64.8 +/- 6.5; P < 0.001 compared with singletons) were similar. The SA of PGDH in reflected chorion leave (46.3 +/- 6.9 nmol/min.mg protein; n = 19) was significantly greater than that in decidua (16 +/- 5.5; n = 15). There was a significant correlation between the levels of PGDH mRNA and PGDH enzyme SA. In fused chorion laeve of diamnionic-dichorionic twin placentae, the SA of PGDH (14.9 +/- 7.3; n = 4) was much less than that in reflected chorion laeve of the same twin pregnancy (70.5 +/- 14.7; n = 4). PGDH mRNA was not detectable in amnion tissue (n = 5) by northern analysis, and the SA of PGDH (< 1.2 +/- 1.0; n = 6) in amnion was undetectable or near the lower limit of assay detection.(ABSTRACT TRUNCATED AT 400 WORDS)