BSD2 is a Rubisco-specific assembly chaperone, forms intermediary hetero-oligomeric complexes, and is nonlimiting to growth in tobacco

BSD2 is a Rubisco-specific assembly chaperone, forms intermediary hetero-oligomeric complexes, and is nonlimiting to growth in tobacco
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DOI:
10.1111/pce.13473
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发表时间:
2019-04-01
影响因子:
7.3
通讯作者:
Whitney, Spencer M.
Whitney, Spencer M.
中科院分区:
生物学1区
文献类型:
--
作者:
Conlan, Brendon;Birch, Rosemary;Whitney, Spencer M.

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Rubisco大小亚基在叶绿体中折叠和组装成L 8 S8全酶涉及许多辅助因素,包括分子伴侣BSD 2。在这里,我们确定了明显的中介Rubisco-BSD 2组装复合物的模型C-3植物烟草。我们发现,BSD 2和Rubisco含量随着叶龄的增加而减少,幼叶中大约一半的BSD 2(类似于70 nmol BSD 2原聚体.m(2))稳定整合在假定的中间Rubisco复合物中,
The folding and assembly of Rubisco large and small subunits into L8S8 holoenzyme in chloroplasts involves many auxiliary factors, including the chaperone BSD2. Here we identify apparent intermediary Rubisco-BSD2 assembly complexes in the model C-3 plant tobacco. We show BSD2 and Rubisco content decrease in tandem with leaf age with approximately half of the BSD2 in young leaves (similar to 70 nmol BSD2 protomer.m(2)) stably integrated in putative intermediary Rubisco complexes that account for