Morphology and phylogenetic position of a mat-forming green plant from acidic rivers in Japan
Morphology and phylogenetic position of a mat-forming green plant from acidic rivers in Japan
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DOI：
10.1007/s10265-003-0125-3
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发表时间：
2003-12-01
影响因子：
2.8
通讯作者：
Nozaki, H
中科院分区：
文献类型：
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作者：
Higuchi, S;Kawamura, M;Nozaki, H
在日本长野县的酸性河流中发现了一种绿色植物，我们将其命名为"Misuzugoke"，在那里它形成宏观垫或聚集体。现场收集的聚集体是近半球形的，由紧凑的分支长丝从中央基地辐射。该植物的细胞含有一个单核和许多盘状叶绿体缺乏蛋白核。由于该植物的简单丝状组织和缺乏生殖器官，它的分类是一个植物或更高的植物不能确定从现场收集的材料。当生长超过3个月的中性琼脂培养基（pH值7.0）含有激素，叶芽特征的brutites出现在植物上。该植物的rbcL基因编码核酮糖-1，5-二磷酸羧化酶/加氧酶大亚基，其部分序列与Dicranella heteromalla（Hedw.）笨蛋（单齿藓亚科，苔藓纲）。基于rbcL基因序列的系统发育分析强烈表明，Misuzugoke位于Haplolpaceae内。这种垫状绿色植物因此被认为是一种简化形式的藓类植物。
A green plant, which we have named "Misuzugoke", was found in acidic rivers in Nagano Prefecture, Japan, where it forms macroscopic mats or aggregates. The field-collected aggregates were nearly hemispherical and were composed of compact branched filaments radiating from a central base. The cells of the plant contained a single nucleus and numerous discoid chloroplasts lacking pyrenoids. Due to the plant's simple filamentous organization and absence of reproductive organs, its classification as an alga or a higher plant could not be determined from the field-collected material. When grown for more than 3 months on neutral agar medium (pH 7.0) containing hormones, leafy shoot buds characteristic of bryophytes appeared on the plant. A partial sequence of the plant's rbcL gene, which encodes the large subunit of ribulose-1, 5-bisphosphate carboxylase/oxygenase was 98% similar to that of Dicranella heteromalla (Hedw.) Schimp. (Haplolepideae, Bryopsida). Phylogenetic analyses based on rbcL gene sequences strongly indicate that Misuzugoke is positioned within the Haplolepideae. This mat-forming green plant is therefore considered to be a reduced form of moss in the Haplolepideae.