Androgen "receptor" in rat liver: cytosol "receptor" deficiency in pseudohermaphrodite male rats.
Androgen "receptor" in rat liver: cytosol "receptor" deficiency in pseudohermaphrodite male rats.
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大鼠肝脏中的雄激素“受体”：假雌雄同体雄性大鼠的细胞质“受体”缺乏。
DOI：
10.1038/newbio242248a0
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发表时间：
1973
期刊：
影响因子：
--
通讯作者：
Arun K. Roy
中科院分区：
文献类型：
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作者：
B. Milin;Arun K. Roy
各种实验系统的证据表明，类固醇激素对靶细胞作用的第一步是激素与特定受体蛋白的结合1-10。因此，可以合理地假设任何激素敏感的靶组织都应含有这些特定的类固醇结合蛋白或“激素受体”。根据我们之前对大鼠肝脏中雄激素依赖性α2U球蛋白合成的研究11-14，肝实质细胞可以被认为是雄激素类固醇的重要靶点。尽管早期报告表明肝组织中没有显着的雄激素结合5，但我们通过改进的检测方法对此进行了研究。我们的结果表明，正常雄性大鼠肝组织的可溶性细胞质部分（胞质溶胶）中存在雄激素受体。发现肝组织的雄激素受体与血清中存在的结合蛋白明显不同，并且雄激素不敏感的假两性雄性大鼠的肝细胞质中实际上不存在该受体。这种突变型大鼠品系中雄激素受体的缺失可能解释了其终末器官雄激素不敏感的分子基础。
EVIDENCE with a variety of experimental systems has indicated that the first step in the action of steroid hormones on target cells is the binding of the hormones with specific receptor proteins1–10. It is reasonable, therefore, to assume that any hormone sensitive target tissue should contain these specific steroid binding proteins or “hormone receptors”. On the basis of our previous work on androgen dependent synthesis of α2U globulin in rat liver11–14, the hepatic parenchymal cells could be considered as an important target for androgenic steroids. In spite of an earlier report indicating no significant androgen binding in the liver tissue5, we studied this with improved detection methods. Our results show the existence of androgen receptor in the soluble cytoplasmic fraction (cytosol) of the liver tissue of normal male rats. The androgen receptor of the hepatic tissue is found to be distinctly different from the binding protein present in the blood serum and the receptor is virtually absent in the liver cytosol of androgen insensitive pseudohermaphrodite male rats. The absence of androgen receptors in this mutant strain of rats might explain the molecular basis for their end organ androgen-insensitivity.