Methyl jasmonate treatment promotes flower opening of cut Eustoma by inducing cell wall loosening proteins in petals
Methyl jasmonate treatment promotes flower opening of cut Eustoma by inducing cell wall loosening proteins in petals
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DOI：
10.1016/j.postharvbio.2013.02.018
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发表时间：
2013-08-01
影响因子：
7
通讯作者：
Yamada, Kunio
中科院分区：
文献类型：
--
作者：
Ochiai, Masaki;Matsumoto, Shogo;Yamada, Kunio
建立控制切花开放率的技术对于维持适当的切花供应以满足消费者需求是重要的。洋桔梗Eustoma grandiflorum（Raf.）希恩保持在含有100 μ M（+/-）-脱落酸（阿坝）、6-苄氨基嘌呤（BA）、赤霉酸-3（GA）、茉莉酸甲酯（MejA）或1-萘乙酸（NAA）的瓶插溶液中。MejA促进花开放。只有开花时间提前，完全开放期的最大花径没有变化。膨胀素和木葡聚糖内糖基转移酶/水解酶（XTH）是一种细胞壁疏松蛋白，参与了洋桔梗从蕾期到完全开放期花瓣的生长。MejA还促进了花瓣中EgEXPA 2、EgEXPA 3和EgXTH 1 mRNA的表达以及expansin和XTH蛋白的积累。同时，阿坝、BA和GA处理均未观察到对花开放和这些基因表达的促进作用。茉莉酸处理后，花瓣细胞壁松弛，扩展素和XTH的表达加快，是茉莉酸处理后提早开花的原因。(C)2013爱思唯尔有限公司版权所有。
Establishing the technique for controlling the rate of cut flower opening is important to maintain appropriate cut flower supplies to meet consumer demand. Cut flowers of Eustoma grandiflorum (Raf.) Shinn. were held in a vase solution containing (+/-)-abscisic acid (ABA), 6-benzylaminopurine (BA), gibberellic acid-3 (GA), methyl jasmonate (MejA) or 1-naphthaleneacetic acid (NAA) at 100 mu M. MejA accelerated flower opening. Only the timing of flowering was earlier, and there was no change in maximum flower diameter at the fully open stage. Expansin and xyloglucan endotransglycosylase/hydrolase (XTH), regarded as cell wall loosing proteins, participate in petal growth from bud stage to the fully open stage in Eustoma. MejA also accelerated the expression of EgEXPA2, EgEXPA3 and EgXTH1 mRNA and the accumulation of expansin and XTH protein in petals. Meanwhile, the acceleration of both flower opening and expression of these genes was not observed by ABA, BA or GA treatment. It was proposed that early flower opening by JA treatment resulted from petal cell wall loosening by accelerated expression of expansin and XTH. (C) 2013 Elsevier B.V. All rights reserved.