Qualitative and quantitative analysis of the active components of the essential oil from Brickellia veronicaefolia by nuclear magnetic resonance spectroscopy
Qualitative and quantitative analysis of the active components of the essential oil from Brickellia veronicaefolia by nuclear magnetic resonance spectroscopy
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DOI：
10.1021/np060180b
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发表时间：
2006-08-25
影响因子：
5.1
通讯作者：
Mata, Rachel
中科院分区：
文献类型：
--
作者：
Rivero-Cruz, Blanca;Rivero-Cruz, Isabel;Mata, Rachel
除了GC-MS分析和HPLC研究之外，还通过NMR光谱法确定了药用物种Brickellia Veronicaefolia的痉挛精油的组成。七个主要化合物，代表CA。86%的油被鉴定为2，6-二甲氧基苯甲酸苄酯（1）、2 '-甲氧基苯甲酸2-羟基苄酯（2）、甘菊环（3）、β-香芹烯（4）、大根香叶烯D（5）、双环大根香叶烯（6）和β-桉叶醇（7）。本文建立了一种灵敏、准确的H-1 NMR分析方法，用于B挥发油中主要化合物的定量分析。婆婆纳该方法使用2，6-二甲氧基苯甲酸苄酯（1）和β-香芹烯（4）进行了验证，这两种活性成分在油中，并成功地应用于测定这些生物活性化合物在三个不同批次的油收集在不同的地理区域和/或季节。
The composition of the spasmolytic essential oil of the medicinal species Brickellia Veronicaefolia was established by NMR spectroscopy in addition to GC-MS analysis and HPLC studies. Seven major compounds, representing ca. 86% of the oil, were identified as benzyl 2,6-dimethoxybenzoate ( 1), 2-hydroxybenzyl 2'-methoxybenzoate ( 2), chamazulene ( 3), beta-caryophyllene (4), germacrene D (5), bicyclogermacrene (6), and beta-eudesmol (7). A sensitive and accurate analytical H-1 NMR method has been developed for the quantification of the major compounds in the essential oil of B. Veronicaefolia. The method was validated using benzyl 2,6-dimethoxybenzoate (1) and beta-caryophyllene (4), two of the active principles in the oil, and successfully applied to the determination of these pharmacologically active compounds in three different batches of the oil collected in different geographical regions and/or seasons.