Multifocal optical-resolution photoacoustic microscopy in vivo.
Multifocal optical-resolution photoacoustic microscopy in vivo.
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DOI：
10.1364/ol.36.001236
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发表时间：
2011-04-01
期刊：
影响因子：
3.6
通讯作者：
Wang LV
中科院分区：
文献类型：
--
作者：
Song L;Maslov K;Wang LV
尽管超声阵列被用于光声成像以提高成像速度，但基于超声阵列的光学分辨率光声显微镜（OR-PAM）以前从未实现过。在这里，我们展示了我们使用微透镜阵列进行光学照明和超声波阵列进行光声检测的多焦点 OR-PAM 的开发。我们的系统能够高速成像体内单个微血管的血红蛋白浓度和氧合。与单焦点相比，多焦点减少了扫描负荷，显着提高了成像速度。当前的多焦点系统可以在 4 分钟内以约 10 μm 横向分辨率获取 1000 × 500 × 200 体素。
Although ultrasound arrays were exploited in photoacoustic imaging to improve imaging speed, ultrasound-array-based optical-resolution photoacoustic microscopy (OR-PAM) has never been achieved previously. Here we present our development of multi-focal OR-PAM using a microlens array for optical illumination and an ultrasound array for photoacoustic detection. Our system is capable of imaging hemoglobin concentration and oxygenation in individual microvessels in vivo at high speed. Compared with a single focus, multiple foci reduce the scanning load and increase the imaging speed significantly. The current multi-focal system can acquire 1000 × 500 × 200 voxels at ~10-μm lateral resolution within 4 min.