Zebrafish deadly seven functions in neurogenesis
Zebrafish deadly seven functions in neurogenesis
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DOI：
10.1006/dbio.2001.0381
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发表时间：
2001-09-15
影响因子：
2.7
通讯作者：
Beattie, CE
中科院分区：
文献类型：
--
作者：
Gray, M;Moens, CB;Beattie, CE
在基因筛选中，我们分离出了一种扰乱运动轴突生长、神经发生和体细胞发生的突变。互补测试表明，这种突变是致命七（des）的等位基因。通过创建遗传嵌合体，我们证明运动轴突缺陷是非细胞自主的。此外，我们发现一些神经嵴细胞群的迁移模式是异常的，并且源自嵴的背根神经节神经元被错位。此外，我们的分析表明，des 突变胚胎表现出神经源性表型。我们发现初级运动神经元的数量和三个后脑网状脊髓神经元的数量增加：Mauthner 细胞、RoL2 细胞和 MiD3cm 细胞。我们还发现 Rohon-Beard 感觉神经元的数量减少，而神经嵴衍生的背根神经节神经元的数量增加，支持先前的假设，即 Rohon-Beard 神经元和神经嵴在发育过程中形成等价组。参与Notch-Delta信号传导的基因突变会导致体细胞发生和神经发生缺陷。我们发现，过度表达Notch的激活形式会减少des突变体中Mauthner细胞的数量，表明des通过Notch-Delta信号通路发挥作用，控制中枢和周围神经系统内特定细胞类型的产生。 (C) 2001 年学术出版社。
In a genetic screen, we isolated a mutation that perturbed motor axon outgrowth, neurogenesis, and somitogenesis. Complementation tests revealed that this mutation is an allele of deadly seven (des). By creating genetic mosaics, we demonstrate that the motor axon defect is non-cell autonomous. In addition, we show that the pattern of migration for some neural crest cell populations is aberrant and crest-derived dorsal root ganglion neurons are misplaced. Furthermore, our analysis reveals that des mutant embryos exhibit a neurogenic phenotype. We find an increase in the number of primary motoneurons and in the number of three hindbrain reticulospinal neurons: Mauthner cells, RoL2 cells, and MiD3cm cells. We also find that the number of Rohon-Beard sensory neurons is decreased whereas neural crest-derived dorsal root ganglion neurons are increased in number supporting a previous hypothesis that Rohon-Beard neurons and neural crest form an equivalence group during development. Mutations in genes involved in Notch-Delta signaling result in defects in somitogenesis and neurogenesis. We found that overexpressing an activated form of Notch decreased the number of Mauthner cells in des mutants indicating that des functions via the Notch-Delta signaling pathway to control the production of specific cell types within the central and peripheral nervous systems. (C) 2001 Academic Press.