The demonstration of a blood-ocular barrier in the albino rat by means of the intravitam deposition of silver.
The demonstration of a blood-ocular barrier in the albino rat by means of the intravitam deposition of silver.
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DOI：
10.1083/jcb.1.6.501
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发表时间：
1955-11-25
期刊：
影响因子：
--
通讯作者：
LADMAN AJ
中科院分区：
文献类型：
--
作者：
WISLOCKI GB;LADMAN AJ
当在大鼠的饮用水中给它们注射硝酸银数月后，它们就会出现全面性银质缺乏。在中枢神经系统中，银的沉积遵循所谓的血脑屏障的模式(Wislocki和Leduc，(2)；Dempsey和Wislocki，(3))。目前的观察涉及到大鼠眼睛中的银沉积，用光学显微镜和电子显微镜进行了研究。在眼睛中，在视网膜的特定神经元素中检测不到银。相反，它大量沉积在睫状突上皮的基底膜和脉络膜毛细血管层与视网膜上皮之间的Bruch基底膜中。用电子显微镜可以在视网膜毛细血管基底膜上看到微量的银，但用光学显微镜无法辨认。在所有这些方面，银的图案类似于它在大脑中的沉积模式。Bruch膜和睫状突中金属的大量聚集类似于在脉络膜神经丛中观察到的，在视网膜毛细血管壁中遇到的痕迹与在大脑毛细血管基底膜中观察到的痕迹相对应。因此，就银而言，眼睛具有类似于血脑屏障的血-眼屏障。银似乎受到睫状突基底膜的屏障的限制，不能进入房水，也不能通过Bruchs基底膜到达视网膜的感光元件，也不能通过视网膜毛细血管周围的基底膜屏障穿透视网膜内层。
When silver nitrate is administered to rats in their drinking water for many months, they develop a generalized argyria. In the central nervous system, the deposition of silver follows the pattern of the so called hematoencephalic barrier (Wislocki and Leduc, (2); Dempsey and Wislocki, (3)). The present observations concern the deposition of silver in the rat's eye, investigated by both light microscopy and the electron microscope. In the eye, silver is not detected in the specific neural elements of the retina. Instead, it is heavily deposited in the basement membrane of the epithelium of the ciliary processes and in Bruch's basal membrane between the choriocapillary layer and the retinal epithelium. Traces of silver are visible in the basement membranes of the retinal capillaries with the electron microscope, but cannot be identified with the light microscope. In all of these respects, the pattern of the silver resembles the mode of its deposition in the brain. The heavy accumulation of metal in Bruch's membrane and the ciliary processes is analogous to that observed in the chorioid plexuses, and the traces encountered in the walls of the retinal capillaries correspond to traces observed in the basement membranes of the cerebral capillaries. Hence, with respect to silver, the eye possesses a blood-ocular barrier similar to the hematoencephalic barrier. Silver appears to be restrained from entering the aqueous humor by a barrier in the basement membrane of the ciliary processes, from reaching the photoreceptor elements of the retina by Bruch's basal membrane, and from penetrating the inner layers of the retina by a barrier in the basement membrane surrounding the retinal capillaries.