Isolation and identification of an iopromide-degrading strain and its application in an A2/O system.
Isolation and identification of an iopromide-degrading strain and its application in an A2/O system.
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DOI：
10.1016/j.biortech.2013.02.043
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发表时间：
2013-04
影响因子：
11.4
通讯作者：
Yanan Liu;Jinlong Hu;Bingjie Xu;Jun He;Pin Gao;Kaiying Liu;G. Xue;S. Ognier
中科院分区：
文献类型：
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作者：
Yanan Liu;Jinlong Hu;Bingjie Xu;Jun He;Pin Gao;Kaiying Liu;G. Xue;S. Ognier
从上海某污水处理厂活性污泥中分离到一株碘普罗胺降解菌。根据其形态特征、生理生化特征和16S rRNA序列的系统发育分析，将该菌鉴定为假单胞菌属(Pseudomonas sp.)24号州际公路。降解IOPr的最佳条件为30℃、pH 7.0。在接种量为5%(V/V)、初始底物为200 mg/L的基础盐培养条件下，菌株I-24对30 mg/L的IOPr降解5d，降解率可达99%。当应用于厌氧-缺氧/好氧(A2/O)工艺时，在其他细菌共存的情况下，菌株I-24对IOPr的去除较低(61.3%)，但在两个A2/O系统(接种和不接种I-24)中，对COD的去除都在95%左右。投加菌株I-24的试验显示，IOPr的去除效果好于未投加的菌株。在实验过程中，I-24在A2/O系统中保持了其丰度。
An iopromide (IOPr)-degrading bacterium was isolated from activated sludge of a wastewater treatment plant in Shanghai. Based on its morphology, physiological-biochemical characteristics and a phylogenetic analysis of its 16S rRNA sequence, the bacterium was identified and named as Pseudomonas sp. I-24. The optimum condition for degrading IOPr was at 30°C and pH 7.0. After 5days, strain I-24 could degrade 30mg/L IOPr by 99% in a basal salts medium with a 5% (V/V) inoculum and 200mg/L starch as the primary substrate. When applied to an Anaerobic-Anoxic/Aerobic (A2/O) process, with the coexistence of other bacteria, the strain I-24 got lower (61.3%) IOPr removal, but in two A2/O systems (with and without I-24 inoculation), the CODcrremoval were both approximately 95%. The trial dosed with strain I-24 showed better IOPr removal than the un-dosed one. I-24 sustained its abundance in the A2/O system during the experiment.