Gene targeting by the TAL effector PthXo2 reveals cryptic resistance gene for bacterial blight of rice.
Gene targeting by the TAL effector PthXo2 reveals cryptic resistance gene for bacterial blight of rice.
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DOI：
10.1111/tpj.12838
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发表时间：
2015-05
期刊：
影响因子：
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通讯作者：
Junhui Zhou;Zhao Peng;Juying Long;Davide Sosso;Bo Liu;J. Eom;Sheng Huang;Sanzhen Liu;C. Vera Cru
中科院分区：
文献类型：
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作者：
Junhui Zhou;Zhao Peng;Juying Long;Davide Sosso;Bo Liu;J. Eom;Sheng Huang;Sanzhen Liu;C. Vera Cru
水稻白叶枯病是由水稻白叶枯病菌Xanthomonas oryzae pv. oryzae，其利用一组III型TAL（转录激活子样）效应子诱导宿主基因表达并调节宿主易感性。有5个SWEET基因在功能上是冗余的，以支持细菌疾病，但只有两个是实验证明的天然TAL效应物的靶点。在此，我们报道了蔗糖转运蛋白基因OsSWEET 13作为PthXo 2的疾病易感基因的鉴定，以及对PthXo 2依赖的X. oryzaepv. OsSWEET 13在粳稻中的启动子变异导致了水稻的耐盐性。含PthXo 2的菌株在籼稻IR 24中诱导OsSWEET 13，这是由于在粳稻Nipponbare和Kitaake的等位基因中不存在一个未预测和未描述的效应子结合位点。水稻品种明恢63的隐性抗病基因xa 25（OsSWEET 13）的一个多态性等位基因也存在单核苷酸多态性（SNP）。利用CRISPR/Cas9技术对OsSWEET 13进行的突变进一步证实了OsSWEET 13的表达对于PthXo 2依赖性疾病对X的易感性状态的需要。oryzaepv.米。对104个菌株的基因图谱分析表明，42个X. oryzaepv.米。OsSWEET 13在本氏烟草叶细胞中的异源表达提高了质外质中的蔗糖浓度。结果证实了一个模型，其中X. oryzaepv.稻提高了蔗糖从宿主细胞的释放，以利用宿主资源。
Bacterial blight of rice is caused by the γ-proteobacterium Xanthomonas oryzae pv. oryzae, which utilizes a group of type III TAL (transcription activator-like) effectors to induce host gene expression and condition host susceptibility. Five SWEET genes are functionally redundant to support bacterial disease, but only two were experimentally proven targets of natural TAL effectors. Here, we report the identification of the sucrose transporter gene OsSWEET13 as the disease-susceptibility gene for PthXo2 and the existence of cryptic recessive resistance to PthXo2-dependent X. oryzae pv. oryzae due to promoter variations of OsSWEET13 in japonica rice. PthXo2-containing strains induce OsSWEET13 in indica rice IR24 due to the presence of an unpredicted and undescribed effector binding site not present in the alleles in japonica rice Nipponbare and Kitaake. The specificity of effector-associated gene induction and disease susceptibility is attributable to a single nucleotide polymorphism (SNP), which is also found in a polymorphic allele of OsSWEET13 known as the recessive resistance gene xa25 from the rice cultivar Minghui 63. The mutation of OsSWEET13 with CRISPR/Cas9 technology further corroborates the requirement of OsSWEET13 expression for the state of PthXo2-dependent disease susceptibility to X. oryzae pv. oryzae. Gene profiling of a collection of 104 strains revealed OsSWEET13 induction by 42 isolates of X. oryzae pv. oryzae. Heterologous expression of OsSWEET13 in Nicotiana benthamiana leaf cells elevates sucrose concentrations in the apoplasm. The results corroborate a model whereby X. oryzae pv. oryzae enhances the release of sucrose from host cells in order to exploit the host resources.