RELATION BETWEEN ORIENTATION OF CENTRAL FIBRILS AND DIRECTION OF BEAT IN CILIA OF OPALINA
RELATION BETWEEN ORIENTATION OF CENTRAL FIBRILS AND DIRECTION OF BEAT IN CILIA OF OPALINA
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DOI：
10.1098/rspb.1970.0020
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发表时间：
1970-01-01
期刊：
影响因子：
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通讯作者：
HORRIDGE, GA
中科院分区：
文献类型：
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作者：
TAMM, SL;HORRIDGE, GA
瞬间固定蝶蛹纤毛活动的异时性波，临界点干燥脱水，扫描电镜观察。用诺马尔斯基干涉对比光学技术拍摄了活体opalina上的波浪照片进行比较。波也在三个不同的平面上切片，用透射电子显微镜检查睫状体的横截面。这是对一些标本从前到后、另一些标本从右到左的异位波进行的。我们的目的是验证中央一对纤毛纤维的轴线控制纤毛跳动方向的理论。波浪的形式和节拍的组成部分是恒定的，无论波浪在动物身上的方向是什么，生物与固定材料的比较表明，形式是忠实地保存下来的。此外，在纤毛的任何区域，无论波浪的方向如何，在恢复和有效击打期间，通过中心对原纤维绘制的线总是与弯曲方向成直角，但这一观察结果本身并不能说明是中心原纤维的平面决定弯曲方向，还是反之亦然。其他考虑得出的结论是，中心原纤维的方向影响有效冲程的方向，但我们不能排除弯曲方向导致中心对被动扭曲的可能性，特别是在恢复冲程期间。
Metachronal waves of ciliary activity onOpalina ranarumwere instantaneously fixed, dehydrated by critical point drying, and examined with the scanning electron microscope. Photographs of waves on livingOpalinawere taken with Nomarski interference-contrast optics for comparison. Waves were also sectioned in three different planes for examination of ciliary cross-sections with the transmission electron microscope. This was done for metachronal waves travelling from anterior to posterior in some specimens and from right to left in others. Our object was to test the theory that the axis of the central pair of ciliary fibrils controls the direction of ciliary beating. The form of the wave and component parts of the beat are constant, no matter what is the direction of the wave across the animal, and comparison of the living with fixed material shows that the form is faithfully preserved. Moreover, in any region of a cilium, a line drawn through the central pair of fibrils is always at right angles to the direction of bending during both recovery and effective strokes, whatever the direction of the wave, but this observation alone does not show whether the plane of the central fibrils determines the direction of bending, or vice versa. Other considerations lead to the conclusion that the orientation of the central fibrils influences the direction of the effective stroke, but we cannot rule out the possibility that the direction of bending causes a passive twist of the central pair, especially during the recovery stroke.