AXONAL MIGRATION OF PROTEINS IN CENTRAL NERVOUS SYSTEM AND PERIPHERAL NERVES AS SHOWN BY RADIOAUTOGRAPHY
AXONAL MIGRATION OF PROTEINS IN CENTRAL NERVOUS SYSTEM AND PERIPHERAL NERVES AS SHOWN BY RADIOAUTOGRAPHY
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DOI：
10.1002/cne.901210304
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发表时间：
1963-01-01
影响因子：
2.5
通讯作者：
LEBLOND, CP
中科院分区：
文献类型：
--
作者：
DROZ, B;LEBLOND, CP
在单次或多次注射标记的氨基酸（蛋氨酸-S35，亮氨酸-或亮氨酸-H3）后间隔长达16天处死的大鼠和小鼠的神经中寻找轴突迁移的证据。脑、脊髓、半月神经节和坐骨神经切片放射自显影。在注射后几分钟内，放射自显影反应出现在核和核周体上，但不在轴突丘或中枢和神经节神经元的轴突本身上。结果表明，神经元的核和核周蛋白质是连续合成的。对核周体中放射性衰变的分析表明，合成了两类蛋白质，一种是缓慢翻转的蛋白质，推测在核周体中被分解代谢，另一种是快速翻转的蛋白质，推测迁移到细胞过程中。在它们出现在核周体后不久，标记的蛋白质可以在轴突丘中找到，然后在轴突中找到。因此，蛋白质从运动神经元和脊髓迁移到坐骨神经轴突。注射后一天，标记蛋白质在轴突中，因为它们穿过白色物质并进入腹根，两天时在腹根中，四天时在腰丛的L5和L 6根的连接处，并且在16天时在坐骨神经的远端约20 mm处。幼鼠和成年鼠的迁移速率分别为1.5mm/d和0.8mm/d。
Evidence of axonal migration was sought in nerves of rats and mice sacrificed at intervals up to 16 days after single or multiple injections of labelled amino acids (methionine-S35, leucine- or arginine-H3). Sections of brain, spinal cord, semilunar ganglion and sciatic nerve were radioautographed. Within minutes after injection, a radioautographic reaction appears over the nucleus and perikaryon, but not over the axon hillock or the axon proper of central and ganglionic neurons. It is concluded that protein is synthesized continuously in the nucleus and perikaryon of neurons. Analysis of the decay of radioactivity in the perikaryon suggests proteins of two catagories are synthesized, a slowly turning over protein, persumably catabolized in the perikaryon, and a rapidly turning over protein presumed to migrate into cell processes. Shortly after their appearance in the perikaryon, the labelled proteins can be found in the axon hillock, and later in the axon. Thus, proteins migrate from the motor neurons and cord into sciatic nerve axons. One day after injection, the labelled protein is in the axons as they cross the white matter and enter the ventral root, at two days well in the ventral root, at four days at the junction of the L5 and L6 roots of the lumbar plexus, and at 16 days about 20 mm more distal in file sciatic nerve. The migration rate is the order of 1.5 mm and 0.8 mm per day in young and adult rats respectively.